A layer

... male gamete has alot of its cytoplasm reduced with a big huge vesicle called the acrosome that contains the enzymes to eat thru the outer slimy layer that surrounds the egg cell. It has concentrated all of its mitocondria to the end where there is the flagella for motility. The egg cell is full ...

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... First the telomeres at each end of the 2 supercoiled chromosomes that fused became sticky by removal of the repeating code that forms a protective layer that makes the ends not-sticky. Then when not-sticky ends are in close enough proximity molecular forces of attraction take over then fusion occurs. ...

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No you need specialized equipment, last I heard one of the labs on campus was using a layer of lipids and then pushing thru the layer perpendicularly to create the micelles. Unfortunately it's a work in progress i.e. no details being divulged.

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... for 10 min in cold room. Then I loaded 2mL of the lysate to 3mL 35%sucrose cushion with dropper. I found that some drops distrubed the sucrose layer surface during the loading process. However, I found that there was still two liquid layers so that I continued the experiment. After 1hr centrifugation ...

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... for 10 min in cold room. Then I loaded 2mL of the lysate to 3mL 35%sucrose cushion with dropper. I found that some drops distrubed the sucrose layer surface during the loading process. However, I found that there was still two liquid layers so that I continued the experiment. After 1hr centrifugation ...

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