IGG1K WESTERN BLOT

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Biologos
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IGG1K WESTERN BLOT

Post by Biologos » Sun Oct 14, 2018 6:33 pm

Dear all,

i an trying to detect an Ab (Igg1k) using an anti-igg1 ab from thermo. When i use dtt or mercaptoethanol i get a single band of either 72-75 kda or around 50 kda. Do i get a breakdown of 1 heavy and 1 light chain (75kda) x2? Or do i only detect heavy chains (around 50kda)?

Your help will be highly appreciated.

Thank you.

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