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DNA sequencing

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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DNA sequencing

Postby roniadam on Mon Feb 25, 2008 10:24 am

Why is it harder to sequence CG rich DNA sequences compared to AT rich ?
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Postby canalon on Mon Feb 25, 2008 8:22 pm

Hint: stablity
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Postby mith on Mon Feb 25, 2008 8:45 pm

remember you need to get a single stranded form of DNA before you can sequence.
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Postby h2so4hurts on Mon Feb 25, 2008 9:29 pm

CG 3 H bonds
AT 2 h bonds

AT is easier to break apart, but if the reaction is hot enough that point is moot :P
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