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Can we autoclave the blotting buffer?

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Can we autoclave the blotting buffer?

Postby ss_kalve on Sat Dec 15, 2007 8:42 am

Can we autoclave the blotting buffer?
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Postby canalon on Sat Dec 15, 2007 6:56 pm

Which buffer? with composittion we could tell if some components are sensitive to heat.
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Postby ss_kalve on Mon Dec 17, 2007 4:28 am

tris base and glycine
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Postby canalon on Tue Dec 18, 2007 3:00 am

As far as I know both can be autoclaved. But the question is : is it necessary to sterilize it?
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Postby ss_kalve on Wed Dec 19, 2007 4:58 am

I think so coz when i used autoclaved running buffer in electrophoresis it was giving good result than nonautoclaved one.so I'm thinking it should also give good result after autoclaving.
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