HELP Failing Biology

[shell]

Please help. I am failing biology and have a homework assignment and cannot find three of the questions. If any one can help I would greatly appreciate it.

1. A biologist isolated a gene from a human cell, attached it to a plasmid, and inserted the plasmid into a bacterium. The plasmid made a new protein, but it was nothing like the protein normally produced in a human cell. Why?

2. Recombinant DNA techniques are used to custom build bacteria for two main purposes: to obtain multiple copies of certain genes and to obtain useful proteins produced by certain genes. Give an example of each of these applications in medicine and agriculture.

3. In the not-too-distant future, gene therapy may be an option for the treatment and cure of some inherited disorders. What do you think are the most serious ethical issues that must be dealt with before human gene therapy is used on a large scale? Why do you think these issues are important?

I know that you guys are not here to do my assignment but I can not figure these three out.

Thanks for any help you can give me..

[mith]

1. no organelles

2.wikipedia

3. will the poor have access?

[AstusAleator]

for number 3, watch the movie Gattaca.

[J20gU3]

Well for one at least humans have RER and Golgi apparatus not just ribosomes in the cytoplasm. The mere fact of a lack of golgi apparatus would change the protein.

[MrMistery]

1. the protein might contain a signal sequence that would have normally been spliced away. Also, it may be due to any posttranslational modifications that might occur in the human cell. However, i think the most obvious differences would come from the presence of introns in human cells.

[mith]

There's also chaperonins etc other folding factors