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DNA Extraction/ Amplication

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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DNA Extraction/ Amplication

Postby Balthowsai on Wed Dec 08, 2004 6:35 pm

Explain why soap solution, table salt and alcohol are used in the extraction of DNA from cheek cells. Reflect on the chemical properties of soap, salt and alcohol.


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Okay, I know the answer has something to do with the polarity of the liquids (polar molecules attracting non-polar, or something like that). However, I can't find the answer anywhere, as to why why the DNA would separate from the cell because of the combination of soap, salt and alcohol. Please help.
Thanks...

Peace.
Balthowsai
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REPLY TO Regarding DNA Extraction/ Amplication:

Postby patna_india on Tue Jan 04, 2005 7:10 pm

HI Balthowsai
i just came across this site today for 1st time and found ur question unanswered.
here is the answer

AS WE KNOW THAT DNA IS STORED IN DOUBLE MEMBRANED NUCLEUS.AND ABOUT THE LIPID CONTENT OF THE MEMBRANE.
WE USE DETERGENT /SOUP SOLUTION TO DISSOLVE THESE MEMBRANE.
SSALT IS USED TO PRECIPITATE THE PROTEINS AND CARBOHYDRATES.
AND LASTLY ALCOHOL IS USED TO GET THE DNA,BY PRECIPITATING IT AND CONTAING IN ITS LAYER.
HOPE U R SATISFIED WITH MY ANSWER
TAHNKS :)
patna_india
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