Real-time PCR&Reverse transcription PCR

[Elva]

Such as subject ,when we see qRT-PCR,what is the really meaning of "RT"?
What are the difference between them?

[mith]

look it up

[JackBean]

RT can be both reverse transcriptase as well as real-time

[qqsvery]

Real-time PCR, also known as kinetic PCR, qPCR, qRT-PCR and RT-qPCR, is quantitative PCR method for the determination of cope number of PCR templates such as DNA or cDNA in a PCR reaction. There are two flavors of real-time PCR: probe-based and intercalator-based.

I`m sure you will be interesting to look into this website to learn more about it！Here it is：
http://www.protocol-online.org/prot/Mol ... index.html

[canalon]

To sum everything up, due to the 2 different possible meaning of RT-PCR, people are gradually shifting to q for quantitative, instead of Real-Time. So it becomes clearer to describe what is done:
qPCR: quantification of DNA
RT-PCR: amplification of RNA
qRT-PCR: quantification of RNA