Can I culture HESCs without feeder cell layer?

[pguo]

Hi,friends,

I have been culturing human embryonic stem cell lines H1 and H9, which were purchased from national stem cell center. I co-culture the cell line with MEF according to the protocol ( http://www.e-biotek.com/ebiotek_010.htm ). I wonder if I can culture them without the feeder cell layer.

You are very appreciated if you can let me know the protocol, or share me your experience.

Thank you