Looking for: Protocol to Culture HESCs without Feeder

[biolearner]

I have been doing experiments with HESCs, H1 and H9 (from national stem cell bank). Right now, I am co-culturing the cell lines with feeder cell line MEF (from ATCC).

I wonder if anyone has the experience to culture H1 and/or H9 without using feeder layer. You are very appreciated if you can let me know the protocol.

Thank you.