interpreting a TEM

[MrMistery]

I know this sounds stupid(mainly because it is)
What might a TEM of a drak circle with a lot of white circles in it represent? I have no idea of the magnification, nor have I ever seen the picture. Any imput would be welcome, although I realize it is next to impossible to deduce anything from this description....

[canalon]

Good guess about the next to impossible to deduce. I guess It would be the image of a round thing that take the stain fairly well with other structures taking it much less on it A scan would be helpful.

I won't be more helpful if you do not guess what my qPCR result curve means without further description

[MrMistery]

Like i said, i have never seen the picture. If I get a copy, i'll upload it

[canalon]

Soyou were offered the same challenge as us: identify a picture on a quite short description? Nice to share with us. OK my guess: A filter wheel for the world smallest qPCR thermocycler, able to work with nanoliter reactions. Yeah for cheap, but hard to pipette

[snowcapk]

White circles = high transmission, so my guess is they are vacuoles or vesicles inside a more electron-dense cell. Mutations in the secretory pathway can cause accumulation of these vesicles (see page three of this pdf).

[cutietech123]

What TEM you mean? Is it Transmission electron microscopy (TEM)? TEM is a microscopy technique whereby a beam of electrons is transmitted through an ultra thin specimen, interacting with the specimen as it passes through it.
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cutie_tech123
I think opinions on wide circles do count.
http://widecirclesworld.wordpress.com

[mith]

Yes, I'm pretty sure that's what he meant.