electrophoresis

[kabuto]

which one is better in gel electrophoresis??
radioactive probe or non-radioactive probe
why??

[mmiaosmiling]

that will depend on what ur aim is and how accurate u need.

[kabuto]

can u explain further?

[canalon]

You do not probe in electrophoresis... you use a probe in a southern. And then radioactivity for probe is almost religion
More seriously some people will defend the non-radiaoctive probes as as good as radioactive, while others will tell you that radioactive is best. In my experience both have problems and at least non-radioactive are much less trouble to handle.

[sachin]

Its best to use non radioactive probe. As intense care to handle and detect.

But non-radioactive or say "fluroscent calating" is best practiced for AGEP, PGAL etc...