Protein phosphorylation

[mrsscientist]

Can anyone help explain the process of Protein phosphorylation please.

[Poison]

I don't know the level of info you need to know about but as a start have a look at this:

http://en.wikipedia.org/wiki/Phosphorylation

For more, make a google search or click the link below. I'm in my good day I made the search for you. ( I know, It isn't hard... ):

http://www.google.com.tr/search?hl=tr&q ... +Ara&meta=

[kk]

My question is regarding mimicing of protein phosphorylation. Changing a serine (Ser) amino acid residue to aspartic acid (Asp) or glutamic acid (Glu) supposedly mimics a phosphorylated serine.

1. How much is it mimicing, can I accept my results based on the presence or Asp/Glu is if my protein was Ser-phosphorylated?

2. Is it simply mimicing because the presence of the -COOH group in Asp/Glu is similar to a phosphate group?

3. Can Asp/Glu mimic threonine (Thr) or tyrosine (Tyr) phosphorylations as well? I guess it could for Thr as well, since Ser and Thr are very similar in shape.

Thanks in advance!