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Improving transient CHO protein expressionModerator: BioTeam
1 post • Page 1 of 1
Improving transient CHO protein expressionI have started using Invitrogen's freestyle CHO cells (without serum, and in suspension) but the protein expression levels are not great compared to the HEKs I was using before.
I have played around with ratios and have an optimal ratio (1:1 with 1ug DNA and 1ug MAX transfection reagent). I have also tried putting the cells in hypothermic conditions which appears to have increased the expression by almost double, but this still isn't quite enough. Any other suggestions of something I can do (without extra cloning) to imrpove the expression of the cells?
1 post • Page 1 of 1
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