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northern blot analysisModerator: BioTeam
3 posts • Page 1 of 1
northern blot analysishi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help...
i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does this mean? thanks in advance
maybe unprocessed and processed RNA?
I have never used northern blots (honestly I didn't know anyone still used them) so I'm just taking a guess here "I have no intention of stopping anytime soon. I want to understand the universe and answer the big questions, that is what keeps me going" - Stephen Hawking
Re: northern blot analysis
Is it possible you are picking up a mRNA from a similar gene that produces a larger transcript?
3 posts • Page 1 of 1
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