Anti-metastasis and Cytostasis, Differentiation between the

[Gemini]

Hi all,

I am currently working on 2 compounds which have shown inhibition of migration when wound healing assay was used. I just wanted to make sure the inhibition was because of the anti-metastatic activity of the compounds, but not because of the cytostatic nature. I am currently using the C6, rat glioma cells and HeLa, human cervical cancer cells to perform the experiment. I have used MTT assay to determine the cytotoxicity of the compounds on the same cell lines.

Can anyone please help me out by giving me an idea of a method to differentiate both of these concepts in vitro. It is very important and also kind of urgent guys .... as my thesis is based on proving this.

Thank you for your help.