Lentivirus vector propagation

[roniadam]

Hi

I bought a lentivirus vector containing alpha globin gene and I want to grow this vector. In what bug I should propagate it?

[MrMistery]

i'm an yeast person, so I am totally out of my element, but I would just look at what species that virus species normally infects and then start from there.

[canalon]

Did the cell (line) in which the virus is effective not come with your virus? That should be basic customer support. I am sure that you are just a phone call (to whoever sold you the lentivirus) to know which cells to use. And by the way lentivirus AFAIK will be used in eukaryotic cell lines, not bacteria.

[MrMistery]

yeah, definitely, probably a cell line from some mammal... I think HIV is a member of the lentiviridae

[canalon]

Yes it is. It is also used as a vector in some case. But this is a large family, hence my answer.