Signaling between the ribosome and synthetase

[moadeeb]

In the translation sequence, there is a point at which the ribosome requires a particular amino acid. Is there a signaling process between the ribosome and Aminoacyl tRNA synthetase indicating a particular need? Or are all varieties of aminoacyl-tRNA floating in the cytoplasm and available for the ribosomes? I hope this is phrased correctly.

[jonmoulton]

As far as I know there is no signaling between ribosome and synthetase. tRNAs linked to amino acids are present in the cytosol. The ribosome uses them, stripping off the amino acids. When an aminoacyl-tRNA synthetase encounters an unloaded tRNA, it puts on an amino acid.

[moadeeb]

No offense but, I'm just not convinced. From what I've heard of the speed and efficiency during many cell processes, I find it hard to believe that simple diffusion of the correct amino acid can account this part of translation. Perhaps the cytoskeleton plays a part.

[MrMistery]

you not believing it doesn't really matter unless you can produce some data to back up your lack of confidence. From what i know, in this particular case it's all diffusion. Don't underestimate diffusion...