Spore counting

[pinkypig]

What is the procedure for visualising spores to perform a total count on them? Taking into account that spores are difficult to stain.

[pinkypig]

can someone help me with this plzzz!!!ineed to know asap!

[pinkypig]

why is nobody repling to this post??is it because no one knows the answer??

[canalon]

For fungus I know they stain with calcofluor white and then count with epifluorescence microscope.
Looking at he litterature other methods: are:
-Heat shock to reactivate the spore and plating and suitable medium
-Haemocytometer, but I guess that must be for eukariotyc parasites

[pinkypig]

No i need to know about staining spores, i have heard somthing about methyl orange and malachite green but not entirely sure,so if anyone knows anymore info about these methods let me know plz!

[Cat]

Here:

http://www.slic2.wsu.edu:82/hurlbert/mi ... 1lab6.html