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Comprative Quant PCR method on RNA questionModerator: BioTeam
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Comprative Quant PCR method on RNA questionI understand that 99% of the RNA isolated with my total RNA isolation kit (qbio problu kit) is rRNA. I am going to quantitate mRNA for specific genes and compare to 16S expression. Do my 16S primers amplify only mRNA for 16S or also all the rRNA? Stupid question maybe but I am confused on some recent studies that indicate over 1000x relative expression to the 16S which should be impossible if rRNA is amplified??
for example: http://aem.asm.org/cgi/content/abstract/73/8/2491 and http://mic.sgmjournals.org/cgi/content/ ... /153/1/102 thank you
1 post • Page 1 of 1
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