wat is ligation mediated PCR??

[yashashri2287]

hey der ppl can ne1 ...pls pls help me by tellin me excatly wat is ligation mediated PCR.... pls help me as soon as possible as i hav my xamz on my head ....

[Cat]

Basically, if you know only part of your sequence and have only one primer, you will need to ligate a linker or adaptor (for the second primer) in order to isolate unknown sequence. It is greatly used in isolation of the insertion flanking sequences in mutants.