Identifying Protiens

[Ciwan]

Hello guys

can someone explain to me how we can identify wherether a protien has a secondary or a globular structure ?

Thanks

[blcr11]

Almost all proteins have secondary structure of some kind--even globular proteins. If you are asking how to tell what kind of structures are there, then you are talking about things like ORD-CD (tells you something about the relative amount of helix vs strands), spectroscopy (noes from nmr tells you something about helices and strands), centrifugation (tells you something about the overall shape). You can always attempt to crystallize a protein and determine its x-ray structure. That way you can actually "see" the secondary, tertiary, and sometimes quaternary structures of a protein or protein complex.

[pluto]

another way to do it is in silicia with prediction software