how to get rid off BSA in the medium

[hisunny4u]

hi every body....
i am culturing Leptospira in EMJH medium containing BSA(very essential)...but leptospira releases Hemolysins...if u try to estimate u get positive result...and one more thing is BSA alone also gives positive result for hemolytic assay ...so my PROBLEM IS HOWCAN I REMOVE BSA FROM THE CULTURE FITRATES(after culturing)..so that it will b easy for estimation...
BSA is also creating problem while running SDS PAGE ...it is producing a big blob in gel ..which is disturbing the visualization of other bands ,...so i want to get rid off BSA in culture filtrates ...and i cannot even skip as it is essential for it ...help me