assessment of cancer growth/invasion, and all that jazz??

[spate]

Hi everyone,

I’m in cancer research and I have currently produced some expression microarray results and I’m now ready for further cellular/molecular studies (which I’m unfamiliar with). I have found some potential genes of interest and I’m testing to see whether they are dependent of another gene (gene A, let say) and its pathway.

So my question is what kind of assays is good to test this hypothesis? I think I will transfect cell lines w/ the potential genes of interest and perform growth/invasion assays, as well as using siRNA to downregulate either the genes of interest or gene A, and see what the effects are. Or maybe I’m not specific enough?

I would like to do more background reading or various assays so any suggestions would be appreciated. Thanks in advance.