This report assesses the variation across fractionated sera processed over a one-month …

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Biology Articles » Methods & Techniques » Laboratory methods to improve SELDI peak detection and quantitation » Conclusion

Conclusion

- Laboratory methods to improve SELDI peak detection and quantitation

In this study, we have investigated the effects of some practical factors for SELDI-TOF analysis of fractionated serum samples. The analysis of 18 spectra (3 batches of samples fractionated 2 weeks apart, 1 samples on each of 6 ProteinChips of one type) independently derived from the same pooled serum sample allowed us to investigate also the compounding effect of reproducibility over time. Reproducibility of mass intensities relies on a high level of standardization and optimization. Our study demonstrates that optimized instrument settings and calibration along with rigorous sample handling and processing can almost double peak detection and substantially decrease the peak intensity CVs. Nevertheless, we feel greater effort is needed to improve peak detection and quantitation and further investigation is needed to assess the reproducibility of the serum fractionation, looking to minimize variation when large sample numbers need to be processed.