The investigators proved that their method was a simple and accurate tool …

• Abstract
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• Materials and methods
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Biology Articles » Methods & Techniques » Improved method of plasma 8-Isoprostane measurement and association analyses with habitual drinking and smoking » Figures

Figures

- Improved method of plasma 8-Isoprostane measurement and association analyses with habitual drinking and smoking

Figure 1 Flow chart of the improved ELISA for plasma 8-isoprostane.

Figure 2 Results of the second extraction step using an NH2 Sep-Pac column to isolate the plasma 8-isoprostane. Spiked plasma samples containing 3H-labeled 8-isoprostane, PGF2α, TXB2, 6-keto-PGF1, PGE2 or PGD2 were used. Samples extracted with ODS gel were used to assess the absorption by, washing and elution from the NH2 Sep-Pac column.

Figure 3 Dilution curves of plasma 8-isoprostane in 3 different plasma samples [(triangle) y = 227.6x-2.34, r = 0.999 ; (square) y = 126.3x + 0.06, r = 1.000; (diamond) y = 21.8x-0.04, r = 0.999].

Figure 4 Changes in AST, ALT, γ-GTP and plasma 8-isoprostane after alcohol intake. The levels of plasma 8-isoprostane in the 3 individuals increase signifi cantly on d 1 after drinking, but return to their original levels on d 2. No significant changes are observed in AST, ALT and γ-GTP. Figure 5 Effect of smoking habit on plasma 8-isoprostane levels. The levels of plasma 8-isoprostane were not signifi cantly different between non smokers and smokers (21.5 ± 7.3 vs 22.8 ± 7.4 ng/L).