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Figure 1. (A) Ovarian cortex section showing: `primordial follicle' (PF), in which the oocyte is encapsulated by flattened pre-granulosa cells; `primary follicle' (F1), in which at least one of the pre-granulosa cells becomes columnar or cubic until to form a single layer of cubic granulosa cells; `secondary follicle' (F2), in which the oocyte is encapsulated by two or more layers of granulosa cells without antrum formation. (B) Aspect of the ovarian cortex of a girl of 12.4 years old (patient no. 11), who had no previous chemotherapy and for whom ovarian tissue was cryopreserved before bone marrow graft for sickle cell disease. Number of primordial and primary follicles/mm2 is 8.5. Scale bar = 75 µm.

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Figure 2. Mean number of primordial and primary follicles/mm2 in the ovarian cortex according to the age of patients and previous chemotherapy.
, no previous chemotherapy;
, previous chemotherapy.

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Source: Human Reproduction, Vol. 17, No. 6, 1447-1452, June 2002.