Authors' contributions
JOA carried out the molecular biology studies, most bioinformatic analyses, and ML phylogenetic analyses and drafted the manuscript. PGF performed the grouped aa Bayesian phylogenetic analyses. RPH provided two cDNA clones and carried out the analyses for 4. RPH and AJR provided advice on analyses and edited the manuscript. All authors read and approved the final manuscript.
Acknowledgements
We thank C. Graham Clark (London School of Hygiene and Tropical Medicine) for generous gifts of cell lysates of three Entamoeba species, T. Martin Embley (University of Newcastle upon Tyne) for sharing the Trichomonas cDNA clones, Erin Gill and Lesley Davis (Dalhousie University) for a generous gift of M. balamuthi genomic DNA and a cDNA sequence, and Miklós Müller (Rockerfeller University) for the cDNA library, Stewart Sarchfield (Dalhousie University) for experimental assistance, Åsa Sjögren (Dalhousie University) for gift of N. gruberi cDNA clones and genomic DNA, experimental assistance and critical reading of the manuscript, and Iñaki Ruiz-Trillo for critical reading of the manuscript. The availability of preliminary sequences from the various sequencing centers is greatly acknowledged.
A.J.R. is supported by a fellowship from the Alfred P. Sloan Foundation, the Peter Lougheed/Canadian Institutes for Health Research New Investigator fellowship, and the Canadian Institute for Advanced Research, Program in Evolutionary Biology. R.P.H. was supported by a Wellcome Trust University Award. This work was supported by a Canadian Institutes of Health Research (CIHR) Grant (MOP-62809) awarded to A.J.R and a Swedish Research Council (VR) Grant awarded to J.O.A.
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