REFERENCES
Abi-Gerges N, Ji GJ, Lu ZJ, Fischmeister R, Hescheler J, and Fleischmann BK. Functional expression and regulation of the hyperpolarization activated non-selective cation current in embryonic stem cell-derived cardiomyocytes. J Physiol 523: 377–389, 2000.
Adams LD, Choi L, Xian HQ, Yang AZ, Sauer B, Wei L, and Gottlieb DI. Double lox targeting for neural cell transgenesis. Mol Brain Res 110: 220–233, 2003.
Alvarez-Dolado M, Pardal R, Garcia-Vardugo JM, Fike JR, Lee HO, Pfeffer K, Lois C, Morrison SJ, and Alvarez-Buylla A. Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes. Nature 425: 968–973, 2003.
Amit M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA, Itskovitz-Eldor J, and Thomson JA. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 227: 271–278, 2000.
Amit M, Margulets V, Segev H, Shariki K, Laevsky I, Coleman R, and Itskovitz-Eldor J. Human feeder layers for human embryonic stem cells. Biol Reprod 68: 2150–2156, 2003.
Andressen C, Stöcker E, Klinz FJ, Lenka N, Hescheler J, Fleischmann B, Arnhold S, and Addicks K. Nestin-specific green fluorescent protein expression in embryonic stem cell-derived neural precursor cells used for transplantation. Stem Cells 19: 419–424, 2001.
Andrews PW. From teratocarcinomas to embryonic stem cells. Philos Trans R Soc Lond B Biol Sci 357: 405–417, 2002.
Angelov DN, Arnhold S, Andressen C, Grabsch H, Puschmann M, Hescheler J, and Addicks K. Temporospatial relationships between macroglia and microglia during in vitro differentiation of murine stem cells. Dev Neurosci 20: 42–51, 1998.
Anisimov SV, Tarasov KV, Riordon D, Wobus AM, and Boheler KR. SAGE identification of differentiation responsive genes in P19 embryonic cells induced to form cardiomyocytes in vitro. Mech Dev 117: 25–74, 2002.
Anisimov SV, Tarasov KV, Tweedie D, Stern MD, Wobus AM, and Boheler KR. SAGE identification of gene transcripts with profiles unique to pluripotent mouse R1 embryonic stem cells. Genomics 79: 169–176, 2002.
Armstrong L, Lako M, Lincoln J, Cairns PM, and Hole N. mTert expression correlates with telomerase activity during the differentiation of murine embryonic stem cells. Mech Dev 97: 109–116, 2000.
Arnhold S, Lenartz D, Kruttwig K, Klinz FJ, Kolossov E, Hescheler J, Sturm V, Andressen C, and Addicks K. Differentiation of green fluorescent protein-labeled embryonic stem cell-derived neural precursor cells into Thy-1-positive neurons and glia after transplantation into adult rat striatum. J Neurosurg 93: 1026–1032, 2000.
Assady S, Maor G, Amit M, Itskovitz-Eldor J, Skorecki KL, and Tzukerman M. Insulin production by human embryonic stem cells. Diabetes 50: 1691–1697, 2001.
Aubert J, Dunstan H, Chambers I, and Smith A. Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation. Nat Biotechnol 20: 1240–1245, 2002.
Aubert J, Stavridis MP, Tweedie S, O'Reilly M, Vierlinger K, Li M, Ghazal P, Pratt T, Mason JO, Roy D, and Smith A. Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice. Proc Natl Acad Sci USA 100: 11836–11841, 2003.
Avilion AA, Nicolis SK, Pevny LH, Perez L, Vivian N, and Lovell-Badge R. Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev 17: 126–140, 2003.
Axelrod HR. Embryonic stem cell lines derived from blastocysts by a simplified technique. Dev Biol 101: 225–228, 1984.
Bader A, Al Dubai H, and Weitzer G. Leukemia inhibitory factor modulates cardiogenesis in embryoid bodies in opposite fashions. Circ Res 86: 787–794, 2000.
Bader A, Gruss A, Hollrigl A, Al Dubai H, Capetanaki Y, and Weitzer G. Paracrine promotion of cardiomyogenesis in embryoid bodies by LIF modulated endoderm. Differentiation 68: 31–43, 2001.
Bagutti C, Wobus AM, Fassler R, and Watt FM. Differentiation of embryonal stem cells into keratinocytes: comparison of wild-type and beta 1 integrin-deficient cells. Dev Biol 179: 184–196, 1996.
Bahramian MB and Zarbl H. Transcriptional and posttranscriptional silencing of rodent alpha 1(I) collagen by a homologous transcriptionally self-silenced transgene. Mol Cell Biol 19: 274–283, 1999.
Bain G, Kitchens D, Yao M, Huettner JE, and Gottlieb DI. Embryonic stem cells express neuronal properties in vitro. Dev Biol 168: 342–357, 1995.
Baker RK, Haendel MA, Swanson BJ, Shambaugh JC, Micales BK, and Lyons GE. In vitro preselection of gene-trapped embryonic stem cell clones for characterizing novel developmentally regulated genes in the mouse. Dev Biol 185: 201–214, 1997.
Banach K, Egert U, and Hescheler J. Excitation spread between heart cells derived form embryonic stem (ES) cells. Pflügers Arch 439: 13–19, 2000.
Barberi T, Klivenyi P, Calingasan NY, Lee H, Kawamata H, Loonam K, Perrier AL, Bruses J, Rubio ME, Topf N, Tabar V, Harrison NL, Beal MF, Moore MAS, and Studer L. Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice. Nat Biotechnol 21: 1200–1207, 2003.
Baron M. Induction of embryonic hematopoietic and endothelial stem/progenitor cells by hedgehog-mediated signals. Differentiation 68: 175–185, 2001.
Bartel FO, Higuchi T, and Spyropoulos DD. Mouse models in the study of the Ets family of transcription factors. Oncogene 19: 6443–6454, 2000.
Bednarik DP, Cook JA, and Pitha PM. Inactivation of the HIV LTR by DNA CpG methylation: evidence for a role in latency. EMBO J 9: 1157–1164, 1990.
Bernstine EG, Hooper ML, Grandchamp S, and Ephrussi B. Alkaline phosphatase activity in mouse teratoma. Proc Natl Acad Sci USA 70: 3899–3903, 1973.
Betts DH, Bordignon V, Hill JR, Winger Q, Westhusin ME, Smith LC, and King WA. Reprogramming of telomerase activity and rebuilding of telomere length in cloned cattle. Proc Natl Acad Sci USA 98: 1077–1082, 2001.
Bhattacharya B, Miura T, Brandenberger R, Mejido J, Luo Y, Yang AX, Joshi BH, Ginis I, Thies RS, Amit M, Lyons I, Condie BG, Itskovitz-Eldor J, Rao MS, and Puri RK. Gene expression in human embryonic stem cell lines: unique molecular signature. Blood 103: 2956–2964, 2004.
Billon N, Jolicoeur C, Tokumoto Y, Vennstrom B, and Raff M. Normal timing of oligodendrocyte development depends on thyroid hormone receptor alpha 1 (TR1). EMBO J 21: 6452–6460, 2002.
Bjornson CR, Rietze RL, Reynolds BA, Magli MC, and Vescovi AL. Turning brain into blood: a hematopoietic fate adopted by adult neural stem cells in vivo. Science 283: 534–537, 1999.
Blau HM, Brazelton TR, and Weimann JM. The evolving concept of a stem cell: entity or function? Cell 105: 829–841, 2001.
Blobel GA, Sieff CA, and Orkin SH. Ligand-dependent repression of the erythroid transcription factor GATA-1 by the estrogen receptor. Mol Cell Biol 15: 3147–3153, 1995.
Blobel GA, Simon MC, and Orkin SH. Rescue of GATA-1-deficient embryonic stem cells by heterologous GATA-binding proteins. Mol Cell Biol 15: 626–633, 1995.
Blyszczuk P, Asbrand C, Rozzo A, Kania G, St-Onge L, Rupnik M, and Wobus AM. Embryonic stem cells differentiate into insulin-producing cells without selection of nestin-expressing cells. Int J Dev Biol 48: 1095–1104, 2004.
Blyszczuk P, Czyz J, Kania G, Wagner M, Roll U, St Onge L, and Wobus AM. Expression of Pax4 in embryonic stem cells promotes differentiation of nestin-positive progenitor and insulin-producing cells. Proc Natl Acad Sci USA 100: 998–1003, 2003.
Boeuf H, Hauss C, Graeve FD, Baran N, and Kedinger C. Leukemia inhibitory factor-dependent transcriptional activation in embryonic stem cells. J Cell Biol 138: 1207–1217, 1997.
Boheler KR, Czyz J, Tweedie D, Yang HT, Anisimov SV, and Wobus AM. Differentiation of pluripotent embryonic stem cells into cardiomyocytes. Circ Res 91: 189–201, 2002.
Boheler KR and Fiszman MY. Can exogenous stem cells be used in transplantation? Cells Tissues Organs 165: 237–245, 1999.
Boheler KR and Stern MD. The new role of SAGE in gene discovery. Trends Biotechnol 21: 55–57, 2003.
Boheler KR and Wobus AM. Myocardial aging and embryonic stem cell biology. In: Advances in Cell Aging and Gerontology in Stem Cells: A Cellular Fountain of Youth, edited by M. P. Mattson and G. van Zant. New York: Elsevier, 2002, vol. 9, chapt. 7, p. 141–177.
Boiani M, Eckardt S, Leu NA, Scholer HR, and McLaughlin KJ. Pluripotency deficit in clones overcome by clone-clone aggregation: epigenetic complementation? EMBO J 22: 5304–5312, 2003.
Bonaldo P, Chowdhury K, Stoykova A, Torres M, and Gruss P. Efficient gene trap screening for novel developmental genes using IRES beta geo vector and in vitro preselection. Exp Cell Res 244: 125–136, 1998.
Bradley A, Evans M, Kaufman MH, and Robertson E. Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines. Nature 309: 255–256, 1984.
Bradley A, Hasty P, Davis A, and Ramirez-Solis R. Modifying the mouse: design and desire. Biotechnology 10: 534–539, 1992.
Brandenberger R, Khrebtukova I, Thies RS, Miura T, Jingli C, Puri R, Vasicek T, Lebkowski J, and Rao M. MPSS profiling of human embryonic stem cells. BMC Dev Biol 4: 10, 2004.
Bremer S, Pellizzer C, Coecke S, Paparella M, and Catalani P. Detection of the embryotoxic potential of cyclophosphamide by using a combined system of metabolic competent cells and embryonic stem cells. ATLA 30: 77–85, 2002.
Bremer S, Worth AP, Paparella M, Bigot K, Kolossov E, Fleischmann BK, Hescheler J, and Balls M. Establishment of an in vitro reporter gene assay for developmental cardiac toxicity. Toxicol In Vitro 15: 215–232, 2001.
Brenin D, Look J, Bader M, Hubner N, Levan G, and Iannaccone P. Rat embryonic stem cells: a progress report. Transplant Proc 29: 1761–1765, 1997.
Brivanlou AH, Gage FH, Jaenisch R, Jessell T, Melton D, and Rossant J. Stem cells. Setting standards for human embryonic stem cells. Science 300: 913–916, 2003.
Brogren CH, Hirsch F, Wood P, Druet P, and Poussier P. Production and characterization of a monoclonal islet cell surface autoantibody from the BB rat. Diabetologia 29: 330–333, 1986.
Brustle O, Jones KN, Learish RD, Karram K, Choudhary K, Wiestler OD, Duncan ID, and McKay RD. Embryonic stem cell-derived glial precursors: a source of myelinating transplants. Science 285: 754–756, 1999.
Brustle O, Spiro AC, Karram K, Choudhary K, Okabe S, and McKay RD. In vitro-generated neural precursors participate in mammalian brain development. Proc Natl Acad Sci USA 94: 14809–14814, 1997.
Buehr M, Nichols J, Stenhouse F, Mountford P, Greenhalgh CJ, Kantachuvesiri S, Brooker G, Mullins J, and Smith AG. Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines. Biol Reprod 68: 222–229, 2003.
Burdon T, Chambers I, Stracey C, Niwa H, and Smith A. Signaling mechanisms regulating self-renewal and differentiation of pluripotent embryonic stem cells. Cells Tissues Organs 165: 131–143, 1999.
Burdon T, Smith A, and Savatier P. Signalling, cell cycle and pluripotency in embryonic stem cells. Trends Cell Biol 12: 432–438, 2002.
Burdon T, Stracey C, Chambers I, Nichols J, and Smith A. Suppression of SHP-2 and ERK signalling promotes self-renewal of mouse embryonic stem cells. Dev Biol 210: 30–43, 1999.
Burns CE and Zon LI. Portrait of a stem cell. Dev Cell 3: 612–613, 2002.
Buttery LD, Bourne S, Xynos JD, Wood H, Hughes FJ, Hughes SP, Episkopou V, and Polak JM. Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells. Tissue Eng 7: 89–99, 2001.
Buzzard JJ, Gough NM, Crook JM, and Colman A. Karyotype of human ES cells during extended culture. Nat Biotechnol 22: 381–382, 2004.
Camenisch G, Gruber M, Donoho G, Van Sloun P, Wenger RH, and Gassmann M. A polyoma-based episomal vector efficiently expresses exogenous genes in mouse embryonic stem cells. Nucleic Acids Res 24: 3707–3713, 1996
Campbell KH, McWhir J, Ritchie WA, and Wilmut I. Sheep cloned by nuclear transfer from a cultured cell line. Nature 380: 64–66, 1996
Cannon JP, Colicos SM, and Belmont JW. Gene trap screening using negative selection: identification of two tandem, differentially expressed loci with potential hematopoietic function. Dev Genet 25: 49–63, 1999.
Capecchi MR. Altering the genome by homologous recombination. Science 244: 1288–1292, 1989.
Capecchi MR. The new mouse genetics: altering the genome by gene targeting. Trends Genet 5: 70–76, 1989.
Caplen NJ. RNAi as a gene therapy approach. Exp Opin Biol Ther 3: 575–586, 2003.
Carpenter MK, Inokuma MS, Denham J, Mujtaba T, Chiu CP, and Rao MS. Enrichment of neurons and neural precursors from human embryonic stem cells. Exp Neurol 172: 383–397, 2001.
Carpenter MK, Rosler ES, Fisk GJ, Brandenberger R, Ares X, Miura T, Lucero M, and Rao MS. Properties of four human embryonic stem cell lines maintained in a feeder-free culture system. Dev Dyn 229: 243–258, 2004.
Cavaleri F and Schöler HR. Nanog. A new recruit to the embryonic stem cell orchestra. Cell 113: 551–557, 2003.
Cervantes RB, Stringer JR, Shao C, Tischfield JA, and Stambrook PJ. Embryonic stem cells and somatic cells differ in mutation frequency and type. Proc Natl Acad Sci USA 99: 3586–3590, 2002.
Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, and Smith A. Functional expression cloning of nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113: 643–655, 2003.
Chang IK, Jeong DK, Hong YH, Park TS, Moon YK, Ohno T, and Han JY. Production of germline chimeric chickens by transfer of cultured primordial germ cells. Cell Biol Int 21: 495–499, 1997.
Chapman G, Remiszewski JL, Webb GC, Schulz TC, Bottema CD, and Rathjen PD. The mouse homeobox gene, Gbx2: genomic organization and expression in pluripotent cells in vitro and in vivo. Genomics 46: 223–233, 1997.
Cherry SR, Biniszkiewicz D, van Parijs L, Baltimore D, and Jaenisch R. Retroviral expression in embryonic stem cells and hematopoietic stem cells. Mol Cell Biol 20: 7419–7426, 2000.
Chien KR. Genes and physiology: molecular physiology in genetically engineered animals. J Clin Invest 97: 901–909, 1996.
Chinzei R, Tanaka Y, Shimizu-Saito K, Hara Y, Kakinuma S, Watanabe M, Teramoto K, Arii S, Takase K, Sato C, Terada N, and Teraoka H. Embryoid-body cells derived from a mouse embryonic stem cell line show differentiation into functional hepatocytes. Hepatology 36: 22–29, 2002.
Chiu CY and Rao MS. Human Embryonic Stem Cells. Totowa, NJ: Humana, 2003.
Choi D, Oh HJ, Chang UJ, Koo SK, Jiang JX, Hwang SY, Lee JD, Yeoh GC, Shin HS, Lee JS, and Oh B. In vivo differentiation of mouse embryonic stem cells into hepatocytes. Cell Transplant 11: 359–368, 2002.
Christoffels VM, Keijser AG, Houweling AC, Clout DE, and Moorman AF. Patterning the embryonic heart: identification of five mouse Iroquois homeobox genes in the developing heart. Dev Biol 224: 263–274, 2000.
Clark AT, Bodnar MS, Fox M, Rodriquez RT, Abeyta MJ, Firpo MT, and Pera RA. Spontaneous differentiation of germ cells from human embryonic stem cells in vitro. Hum Mol Genet 13: 727–739, 2004.
Clarke DL, Johansson CB, Wilbertz J, Veress B, Nilsson E, Karlstrom H, Lendahl U, and Frisen J. Generalized potential of adult neural stem cells. Science 288: 1660–1663, 2000.
Colucci-Guyon E, Portier MM, Dunia I, Paulin D, Pournin S, and Babinet C. Mice lacking vimentin develop and reproduce without an obvious phenotype. Cell 79: 679–694, 1994.
Copeland NG, Jenkins NA, and Court DL. Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet 2: 769–779, 2001.
Coraux C, Hilmi C, Rouleau M, Spadafora A, Hinnrasky J, Ortonne JP, Dani C, and Aberdam D. Reconstituted skin from murine embryonic stem cells. Curr Biol 13: 849–853, 2003.
Cowan CA, Klimanskaya I, McMahon J, Atienza J, Witmyer J, Zucker JP, Wang S, Morton CC, McMahon AP, Powers D, and Melton DA. Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med 350: 1353–1356, 2004.
Czyz J, Guan K, Zeng Q, Nikolova T, Meister A, Schonborn F, Schuderer J, Kuster N, and Wobus AM. High frequency electromagnetic fields (GSM signals) affect gene expression levels in tumor suppressor p53-deficient embryonic stem cells. Bioelectromagnetics 25: 296–307, 2004.
Czyz J, Nikolova T, Schuderer J, Kuster N, and Wobus AM. Non-thermal effects of power-line magnetic fields (50 Hz) on gene expression levels of pluripotent embryonic stem cells: the role of tumour suppressor p53. Mutat Res 557: 63–74, 2004.
Czyz J and Wobus A. Embryonic stem cell differentiation: the role of extracellular factors. Differentiation 68: 167–174, 2001.
Daheron L, Opitz SL, Zaehres H, Lensch WM, Andrews PW, Itskovitz-Eldor J, and Daley GQ. LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells. Stem Cells 22: 770–778, 2004.
Dang SM and Zandstra PW. Scalable production of embryonic stem cell-derived cells. Methods Mol Biol 290: 353–364, 2004.
Dani C, Smith AG, Dessolin S, Leroy P, Staccini L, Villageois P, Darimont C, and Ailhaud G. Differentiation of embryonic stem cells into adipocytes in vitro. J Cell Sci 110: 1279–1285, 1997.
Davila JC, Cezar GG, Thiede M, Strom S, Miki T, and Trosko J. Use and application of stem cells in toxicology. Toxicol Sci 79: 214–223, 2004.
Dinsmore J, Ratliff J, Deacon T, Pakzaban P, Jacoby D, Galpern W, and Isacson O. Embryonic stem cells differentiated in vitro as a novel source of cells for transplantation. Cell Transplant 5: 131–143, 1996.
Do JT and Schöler HR. Nuclei of embryonic stem cells reprogram somatic cells. Stem Cells 22: 941–949, 2004.
Doetschman T, Williams P, and Maeda N. Establishment of hamster blastocyst-derived embryonic stem (ES) cells. Dev Biol 127: 224–227, 1988.
Doetschman TC, Eistetter H, Katz M, Schmidt W, and Kemler R. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J Embryol Exp Morphol 87: 27–45, 1985.
Drab M, Haller H, Bychkov R, Erdmann B, Lindschau C, Haase H, Morano I, Luft FC, and Wobus AM. From totipotent embryonic stem cells to spontaneously contracting smooth muscle cells: a retinoic acid and db-cAMP in vitro differentiation model. FASEB J 11: 905–915, 1997.
Draper JS, Smith K, Gokhale P, Moore HD, Maltby E, Johnson J, Meisner L, Zwaka TP, Thomson JA, and Andrews PW. Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat Biotechnol 22: 53–54, 2004.
Drukker M, Katz G, Urbach A, Schuldiner M, Markel G, Itskovitz-Eldor J, Reubinoff B, Mandelboim O, and Benvenisty N. Characterization of the expression of MHC proteins in human embryonic stem cells. Proc Natl Acad Sci USA 99: 9864–9869, 2002.
Durick K, Mendlein J, and Xanthopoulos KG. Hunting with traps: genome-wide strategies for gene discovery and functional analysis. Genome Res 9: 1019–1025, 1999.
Dvash T, Mayshar Y, Darr H, McElhaney M, Barker D, Yanuka O, Kotkow KJ, Rubin LL, Benvenisty N, and Eiges R. Temporal gene expression during differentiation of human embryonic stem cells and embryoid bodies. Hum Reprod. In press.
Eiges R, Schuldiner M, Drukker M, Yanuka O, Itskovitz-Eldor J, and Benvenisty N. Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells. Curr Biol 11: 514–518, 2001.
Eisenbarth GS, Shimizu K, Bowring MA, and Wells S. Expression of receptors for tetanus toxin and monoclonal antibody A2B5 by pancreatic islet cells. Proc Natl Acad Sci USA 79: 5066–5070, 1982.
Elliott ST, Crider DG, Garham CP, Boheler KR, and Van Eyk JE. Two-dimensional gel electrophoresis database of murine R1 embryonic stem cells. Proteomics. 4: 3813–3832, 2004.
Evans MJ. The isolation and properties of a clonal tissue culture strain of pluripotent mouse teratoma cells. J Embryol Exp Morphol 28: 163–176, 1972.
Evans MJ and Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature 292: 154–156, 1981.
Evsikov AV and Solter D. Comment on "Stemness": transcriptional profiling of embryonic and adult stem cells" and "a stem cell molecular signature." Science 302: 393, 2003.
Faendrich F, Lin X, Chai GX, Schulze M, Ganten D, Bader M, Holle J, Huang DS, Parwaresch R, Zavazava N, and Binas B. Preimplantation-stage stem cells induce long-term allogeneic graft acceptance without supplementary host conditioning. Nat Med 8: 171–178, 2002.
Fairchild PJ, Brook FA, Gardner RL, Graca L, Strong V, Tone Y, Tone M, Nolan KF, and Waldmann H. Directed differentiation of dendritic cells from mouse embryonic stem cells. Curr Biol 10: 1515–1518, 2000.
Fan Y, Melhem MF, and Chaillet JR. Forced expression of the homeobox-containing gene Pem blocks differentiation of embryonic stem cells. Dev Biol 210: 481–496, 1999.
Fassler R, Rohwedel J, Maltsev V, Bloch W, Lentini S, Guan K, Gullberg D, Hescheler J, Addicks K, and Wobus AM. Differentiation and integrity of cardiac muscle cells are impaired in the absence of beta 1 integrin. J Cell Sci 109: 2989–2999, 1996.
Fehling HJ, Lacaud G, Kubo A, Kennedy M, Robertson S, Keller G, and Kouskoff V. Tracking mesoderm induction and its specification to the hemangioblast during embryonic stem cell differentiation. Development 130: 4217–4227, 2003.
Fijnvandraat AC, van Ginneken AC, Schumacher CA, Boheler KR, Lekanne Deprez RH, Christoffels VM, and Moorman AF. Cardiomyocytes purified from differentiated embryonic stem cells exhibit characteristics of early chamber myocardium. J Mol Cell Cardiol 35: 1461–1472, 2003.
Finley MF, Kulkarni N, and Huettner JE. Synapse formation and establishment of neuronal polarity by P19 embryonic carcinoma cells and embryonic stem cells. J Neurosci 16: 1056–1065, 1996.
Fire A, Xu SQ, Montgomery MK, Kostas SA, Driver SE, and Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806–811, 1998.
Fischer M, Goldschmitt J, Peschel C, Brakenhoff JP, Kallen KJ, Wollmer A, Grotzinger J, and Rose-John SI. A bioactive designer cytokine for human hematopoietic progenitor cell expansion. Nat Biotechnol 15: 142–145, 1997.
Fodor W. Tissue engineering and cell based therapies, from the bench to the clinic: the potential to replace, repair and regenerate. Reprod Biol Endocrinol 1: 102, 2003.
Forrester LM, Nagy A, Sam M, Watt A, Stevenson L, Bernstein A, Joyner AL, and Wurst W. An induction gene trap screen in embryonic stem cells: identification of genes that respond to retinoic acid in vitro. Proc Natl Acad Sci USA 93: 1677–1682, 1996.
Fortunel NO, Otu HH, Ng HH, Chen JH, Mu XQ, Chevassut T, Li XY, Joseph M, Bailey C, Hatzfeld JA, Hatzfeld A, Usta F, Vega VB, Long PM, Libermann TA, and Lim B. Comment on "Stemness": transcriptional profiling of embryonic and adult stem cells" and "A stem cell molecular signature" (I). Science 302: 393, 2003.
Fraichard A, Chassande O, Bilbaut G, Dehay C, Savatier P, and Samarut J. In vitro differentiation of embryonic stem cells into glial cells and functional neurons. J Cell Sci 108: 3181–3188, 1995.
Franco D, Campione M, Kelly R, Zammit PS, Buckingham M, Lamers WH, and Moorman AFM. Multiple transcriptional domains, with distinct left and right components, in the atrial chambers of the developing heart. Circ Res 87: 984–991, 2000.
Franco D, Lamers WH, and Moorman AF. Patterns of expression in the developing myocardium: towards a morphologically integrated transcriptional model. Cardiovasc Res 38: 25–53, 1998.
Franke WW, Grund C, Kuhn C, Jackson BW, and Illmensee K. Formation of cytoskeletal elements during mouse embryogenesis. III. Primary mesenchymal cells and the first appearance of vimentin filaments. Differentiation 23: 43–59, 1982.
Franke WW, Schmid E, Winter S, Osborn M, and Weber K. Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates. Exp Cell Res 123: 25–46, 1979.
Fraser ST, Ogawa M, Nishikawa S, and Nishikawa S. Embryonic stem cell differentiation as a model to study hematopoietic and endothelial cell development. Methods Mol Biol 185: 71–81, 2002.
Freed CR, Greene PE, Breeze RE, Tsai WY, DuMouchel W, Kao R, Dillon S, Winfield H, Culver S, Trojanowski JQ, Eidelberg D, and Fahn S. Transplantation of embryonic dopamine neurons for severe Parkinson's disease. N Engl J Med 344: 710–719, 2001.
Friedrich G and Soriano P. Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev 5: 1513–1523, 1991.
Fuchs E and Byrne C. The epidermis: rising to the surface. Curr Opin Genet Dev 4: 725–736, 1994.
Fuchs E and Segre JA. Stem cells: a new lease on life. Cell 100: 143–155, 2000.
Gage FH. Cell therapy. Nature 392: 18–24, 1998.
Galli R, Borello U, Gritti A, Minasi MG, Bjornson C, Coletta M, Mora M, De Angelis MG, Fiocco R, Cossu G, and Vescovi AL. Skeletal myogenic potential of human and mouse neural stem cells. Nat Neurosci 3: 986–991, 2000.
Gassmann M, Donoho G, and Berg P. Maintenance of an extrachromosomal plasmid vector in mouse embryonic stem cells. Proc Natl Acad Sci USA 92: 1292–1296, 1995.
Gearhart JD and Mintz B. Contact-mediated myogenesis and increased acetylcholinesterase activity in primary cultures of mouse teratocarcinoma cells. Proc Natl Acad Sci USA 71: 1734–1738, 1974.
Geijsen N, Horoschak M, Kim K, Gribnau J, Eggan K, and Daley GQ. Derivation of embryonic germ cells and male gametes from embryonic stem cells. Nature 2003.
Genschow E, Scholz G, Brown N, Piersma A, Brady M, Clemann N, Huuskonen H, Paillard F, Bremer S, Becker K, and Spielmann H. Development of prediction models for three in vitro embryotoxicity tests in an ECVAM validation study. In Vitro Mol Toxicol 13: 51–66, 2000.
Ginis I, Luo Y, Miura T, Thies S, Brandenberger R, Gerecht-Nir S, Amit M, Hoke A, Carpenter MK, Itskovitz-Eldor J, and Rao MS. Differences between human and mouse embryonic stem cells. Dev Biol 269: 360–380, 2004.
Goldstein RS, Drukker M, Reubinoff BE, and Benvenisty N. Integration and differentiation of human embryonic stem cells transplanted to the chick embryo. Dev Dyn 225: 80–86, 2002.
Gossler A, Joyner AL, Rossant J, and Skarnes WC. Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244: 463–465, 1989.
Gottlieb DI. Large-scale sources of neural stem cells. Annu Rev Neurosci 25: 381–407, 2002.
Graves KH and Moreadith RW. Derivation and characterization of putative pluripotential embryonic stem cells from preimplantation rabbit embryos. Mol Reprod Dev 36: 424–433, 1993.
Gropp M, Itsykson P, Singer O, Ben-Hur T, Reinhartz E, Galun E, and Reubinoff BE. Stable genetic modification of human embryonic stem cells by lentiviral vectors. Mol Ther 7: 281–287, 2003.
Grusby MJ and Glimcher LH. Immune-responses in MHC class II-deficient mice. Annu Rev Immunol 13: 417–435, 1995.
Guan K, Czyz J, Furst DO, and Wobus AM. Expression and cellular distribution of alpha(v)integrins in beta(1)integrin-deficient embryonic stem cell-derived cardiac cells. J Mol Cell Cardiol 33: 521–532, 2001.
Guan K, Furst DO, and Wobus AM. Modulation of sarcomere organization during embryonic stem cell-derived cardiomyocyte differentiation. Eur J Cell Biol 78: 813–823, 1999.
Gupta S. Hepatocyte transplantation. J Gastroenterol Hepatol 17: 287–293, 2002.
Gussoni E, Soneoka Y, Strickland CD, Buzney EA, Khan MK, Flint AF, Kunkel LM, and Mulligan RC. Dystrophin expression in the mdx mouse restored by stem cell transplantation. Nature 401: 390–394, 1999.
Hamazaki T, Iiboshi Y, Oka M, Papst PJ, Meacham AM, Zon LI, and Terada N. Hepatic maturation in differentiating embryonic stem cells in vitro. FEBS Lett 497: 15–19, 2001.
Helgason CD, Sauvageau G, Lawrence HJ, Largman C, and Humphries RK. Overexpression of HOXB4 enhances the hematopoietic potential of embryonic stem cells differentiated in vitro. Blood 87: 2740–2749, 1996.
Henderson JK, Draper JS, Baillie HS, Fishel S, Thomson JA, Moore H, and Andrews PW. Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329–337, 2002.
Hernandez L, Kozlov S, Piras G, and Stewart CL. Paternal and maternal genomes confer opposite effects on proliferation, cell-cycle length, senescence, and tumor formation. Proc Natl Acad Sci USA 100: 13344–13349, 2003.
Herrera PL, Nepote V, and Delacour A. Pancreatic cell lineage analyses in mice. Endocrine 19: 267–277, 2002.
Hescheler J, Fleischmann BK, Lentini S, Maltsev VA, Rohwedel J, Wobus AM, and Addicks K. Embryonic stem cells: a model to study structural and functional properties in cardiomyogenesis. Cardiovasc Res 36: 149–162, 1997.
Hescheler J, Wartenberg M, Fleischmann BK, Banach K, Acker H, and Sauer H. Embryonic stem cells as a model for the physiological analysis of the cardiovascular system. Methods Mol Biol 185: 169–187, 2002.
Hess D, Li L, Martin M, Sakano S, Hill D, Strutt B, Thyssen S, Gray DA, and Bhatia M. Bone marrow-derived stem cells initiate pancreatic regeneration. Nat Biotechnol 21: 763–770, 2003.
Hidaka M, Caruana G, Stanford WL, Sam M, Correll PH, and Bernstein A. Gene trapping of two novel genes, Hzf and Hhl, expressed in hematopoietic cells. Mech Dev 90: 3–15, 2000.
Hochedlinger K and Jaenisch R. Nuclear transplantation, embryonic stem cells, and the potential for cell therapy. N Engl J Med 349: 275–286, 2003.
Hole N. Embryonic stem cell-derived haematopoiesis. Cells Tissues Organs 165: 181–189, 1999.
Hole N, Graham GJ, Menzel U, and Ansell JD. A limited temporal window for the derivation of multilineage repopulating hematopoietic progenitors during embryonal stem cell differentiation in vitro. Blood 88: 1266–1276, 1996.
Holmes TC. Novel peptide-based biomaterial scaffolds for tissue engineering. Trends Biotechnol 20: 16–21, 2002.
Hori Y, Rulifson IC, Tsai BC, Heit JJ, Cahoy JD, and Kim SK. Growth inhibitors promote differentiation of insulin-producing tissue from embryonic stem cells. Proc Natl Acad Sci USA 99: 16105–16110, 2002.
Hosler BA, Rogers MB, Kozak CA, and Gudas LJ. An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells. Mol Cell Biol 13: 2919–2928, 1993.
Hübner K, Fuhrmann G, Christenson LK, Kehler J, Reinbold R, De La FR, Wood J, Strauss IIIJF, Boiani M, and Schöler HR. Derivation of oocytes from mouse embryonic stem cells. Science 300: 1251–1256, 2003.
Hwang WS, Ryu YJ, Park JH, Park ES, Lee EG, Koo JM, Jeon HY, Lee BC, Kang SK, Kim SJ, Ahn C, Hwang JH, Park KY, Cibelli JB, and Moon SY. Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst. Science 303: 1669–1674, 2004.
Iannaccone PM, Taborn GU, Garton RL, Caplice MD, and Brenin DR. Pluripotent embryonic stem cells from the rat are capable of producing chimeras. Dev Biol 163: 288–292, 1994.
Igelmund P, Fleischmann BK, Fischer IR, Soest J, Gryshchenko O, Bohm-Pinger MM, Sauer H, Liu Q, and Hescheler J. Action potential propagation failures in long-term recordings from embryonic stem cell-derived cardiomyocytes in tissue culture. Pflügers Arch 437: 669–679, 1999.
Itskovitz-Eldor J, Schuldiner M, Karsenti D, Eden A, Yanuka O, Amit M, Soreq H, and Benvenisty N. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med 6: 88–95, 2000.
Ivanova NB, Dimos JT, Schaniel C, Hackney JA, Moore KA, and Lemischka IR. A stem cell molecular signature. Science 298: 601–604, 2002.
Jackson KA, Mi T, and Goodell MA. Hematopoietic potential of stem cells isolated from murine skeletal muscle. Proc Natl Acad Sci USA 96: 14482–14486, 1999.
Jahner D, Stuhlmann H, Stewart CL, Harbers K, Lohler J, Simon I, and Jaenisch R. De novo methylation and expression of retroviral genomes during mouse embryogenesis. Nature 298: 623–628, 1982.
Jaisser F and Beggah AT. Transgenic models in renal tubular physiology. Exp Nephrol 6: 438–446, 1998.
Jakob H, Boon T, Gaillard J, Nicolas JF, and Jacob F. Teratocarcinoma of the mouse: isolation, culture and properties of pluripotential cells. Ann Microbiol 124: 269–282, 1973.
Ji GJ, Fleischmann BK, Bloch W, Feelisch M, Andressen C, Addicks K, and Hescheler J. Regulation of the L-type Ca2+ channel during cardiomyogenesis: switch from NO to adenylyl cyclase-mediated inhibition. FASEB J 13: 313–324, 1999.
Jiang Y, Vaessen B, Lenvik T, Blackstad M, Reyes M, and Verfaillie CM. Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain. Exp Hematol 30: 896–904, 2002.
Johansson BM and Wiles MV. Evidence for involvement of activin A and bone morphogenetic protein 4 in mammalian mesoderm and hematopoietic development. Mol Cell Biol 15: 141–151, 1995.
Jones EA, Tosh D, Wilson DI, Lindsay S, and Forrester LM. Hepatic differentiation of murine embryonic stem cells. Exp Cell Res 272: 15–22, 2002.
Jones-Villeneuve EM, McBurney MW, Rogers KA, and Kalnins VI. Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells. J Cell Biol 94: 253–262, 1982.
Joyner AL. Gene targeting and gene trap screens using embryonic stem cells: new approaches to mammalian development. Bioessays 13: 649–656, 1991.
Kahan BW and Ephrussi B. Developmental potentialities of clonal in vitro cultures of mouse testicular teratoma. J Natl Cancer Inst 44: 1015–1036, 1970.
Kania G, Blyszczuk P, Czyz J, Navarrete-Santos A, and Wobus AM. Differentiation of mouse embryonic stem cells into pancreatic and hepatic cells. In: Methods Enzymology (365th ed.), edited by P. M. Wassarman and G. M. Keller. 2003, chapt. 21, p. 287–302.
Kania G, Blyszczuk P, Jochheim A, Ott M, and Wobus AM. Generation of glycogen and albumin producing hepatocyte-like cells from embryonic stem cells. Biol Chem 385: 943–953, 2004.
Kania G, Corbeil D, Tarasov KV, Blyszczuk P, Huttner WB, Boheler KR, and Wobus AM. The somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors. Stem Cells. In press.
Kaufman DS, Lewis RL, Hanson ET, Auerbach R, Plendl J, and Thomson JA. Functional endothelial cells derived from rhesus monkey embryonic stem cells. Blood: 2003, 2003.
Kaufman DS and Thomson JA. Human ES cells: haematopoiesis and transplantation strategies. J Anat 200: 243–248, 2002.
Kawasaki H, Mizuseki K, Nishikawa S, Kaneko S, Kuwana Y, Nakanishi S, Nishikawa SI, and Sasai Y. Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity. Neuron 28: 31–40, 2000.
Kawasaki H, Suemori H, Mizuseki K, Watanabe K, Urano F, Ichinose H, Haruta M, Takahashi M, Yoshikawa K, Nishikawa SI, Nakatsuji N, and Sasai Y. Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity. Proc Natl Acad Sci USA 99: 1580–1585, 2002.
Kehat I, Kenyagin-Karsenti D, Snir M, Segev H, Amit M, Gepstein A, Livne E, Binah O, Itskovitz-Eldor J, and Gepstein L. Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J Clin Invest 108: 407–414, 2001.
Keller GM. In vitro differentiation of embryonic stem cells. Curr Opin Cell Biol 7: 862–869, 1995.
Kerr DA, Llado J, Shamblott MJ, Maragakis NJ, Irani DN, Crawford TO, Krishnan C, Dike S, Gearhart JD, and Rothstein JD. Human embryonic germ cell derivatives facilitate motor recovery of rats with diffuse motor neuron injury. J Neurosci 23: 5131–5140, 2003.
Kim JH, Auerbach JM, Rodriguez-Gomez JA, Velasco I, Gavin D, Lumelsky N, Lee SH, Nguyen J, Sanchez-Pernaute R, Bankiewicz K, and McKay R. Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease. Nature 418: 50–56, 2002.
Klug MG, Soonpaa MH, Koh GY, and Field LJ. Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts. J Clin Invest 98: 216–224, 1996.
Kolossov E, Fleischmann BK, Liu Q, Bloch W, Viatchenko-Karpinski S, Manzke O, Ji GJ, Bohlen H, Addicks K, and Hescheler J. Functional characteristics of ES cell-derived cardiac precursor cells identified by tissue-specific expression of the green fluorescent protein. J Cell Biol 143: 2045–2056, 1998.
Kramer J, Hegert C, Guan K, Wobus AM, Muller PK, and Rohwedel J. Embryonic stem cell-derived chondrogenic differentiation in vitro: activation by BMP-2 and BMP-4. Mech Dev 92: 193–205, 2000.
Kunkel TA and Bebenek K. DNA replication fidelity. Annu Rev Biochem 69: 497–529, 2000.
Kyba M, Perlingeiro RC, and Daley GQ. HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors. Cell 109: 29–37, 2002.
Labosky PA, Barlow DP, and Hogan BL. Embryonic germ cell lines and their derivation from mouse primordial germ cells. Ciba Found Symp 182: 157–168, 1994a.
Labosky PA, Barlow DP, and Hogan BL. Mouse embryonic germ (EG) cell lines: transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines. Development 120: 3197–3204, 1994.
Lako M, Lindsay S, Lincoln J, Cairns PM, Armstrong L, and Hole N. Characterisation of Wnt gene expression during the differentiation of murine embryonic stem cells in vitro: role of Wnt3 in enhancing haematopoietic differentiation. Mech Dev 103: 49–59, 2001.
Langer R. Tissue engineering. Mol Ther 1: 12–15, 2000.
Lanza RP, Cibelli JB, and West MD. Human therapeutic cloning. Nat Med 5: 975–977, 1999.
Lavon N and Benvenisty N. Differentiation and genetic manipulation of human embryonic stem cells and the analysis of the cardiovascular system. Trends Cardiovasc Med 13: 47–52, 2003.
Lavon N, Yanuka O, and Benvenisty N. Differentiation and isolation of hepatic-like cells from human embryonic stem cells. Differentiation 72: 230–238, 2004.
Leahy A, Xiong JW, Kuhnert F, and Stuhlmann H. Use of developmental marker genes to define temporal and spatial patterns of differentiation during embryoid body formation. J Exp Zool 284: 67–81, 1999.
Lee JB, Lee JE, Park JH, Kim SJ, Kim MK, Roh SI, and Yoon HS. Establishment and maintenance of human embryonic stem cell lines on human feeder cells derived from uterine endometrium under serum-free condition. Biol Reprod. In press.
Lee SH, Lumelsky N, Studer L, Auerbach JM, and McKay RD. Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells. Nat Biotechnol 18: 675–679, 2000.
Leon-Quinto T, Jones J, Skoudy A, Burcin M, and Soria B. In vitro directed differentiation of mouse embryonic stem cells into insulin-producing cells. Diabetologia 47: 1442–1451, 2004.
Levenberg S, Golub JS, Amit M, Itskovitz-Eldor J, and Langer R. Endothelial cells derived from human embryonic stem cells. Proc Natl Acad Sci USA 99: 4391–4396, 2002.
Lewandoski M. Conditional control of gene expression in the mouse. Nature Rev Genet 2: 743–755, 2001.
Li M, Pevny L, Lovell-Badge R, and Smith A. Generation of purified neural precursors from embryonic stem cells by lineage selection. Curr Biol 8: 971–974, 1998.
Liu S, Qu Y, Stewart TJ, Howard MJ, Chakrabortty S, Holekamp TF, and McDonald JW. Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation. Proc Natl Acad Sci USA 97: 6126–6131, 2000.
Lo L, Sommer L, and Anderson DJ. MASH1 maintains competence for BMP2-induced neuronal differentiation in post-migratory neural crest cells. Curr Biol 7: 440–450, 1997.
Lumelsky N, Blondel O, Laeng P, Velasco I, Ravin R, and McKay R. Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets. Science 292: 1389–1394, 2001.
Ma Y, Ramezani A, Lewis R, Hawley RG, and Thomson JA. High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors. Stem Cells 21: 111–117, 2003.
Makino S, Fukuda K, Miyoshi S, Konishi F, Kodama H, Pan J, Sano M, Takahashi T, Hori S, Abe H, Hata J, Umezawa A, and Ogawa S. Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest 103: 697–705, 1999.
Maltsev VA, Ji GJ, Wobus AM, Fleischmann BK, and Hescheler J. Establishment of beta-adrenergic modulation of L-type Ca2+ current in the early stages of cardiomyocyte development. Circ Res 84: 136–145, 1999.
Maltsev VA, Rohwedel J, Hescheler J, and Wobus AM. Embryonic stem cells differentiate in vitro into cardiomyocytes representing sinusnodal, atrial and ventricular cell types. Mech Dev 44: 41–50, 1993.
Maltsev VA, Wobus AM, Rohwedel J, Bader M, and Hescheler J. Cardiomyocytes differentiated in vitro from embryonic stem cells developmentally express cardiac-specific genes and ionic currents. Circ Res 75: 233–244, 1994.
Mangi AA, Noiseux N, Kong DL, He HM, Rezvani M, Ingwall JS, and Dzau VJ. Mesenchymal stem cells modified with Akt prevent remodeling and restore performance of infarcted hearts. Nature Med 9: 1195–1201, 2003.
Marchetti S, Gimond C, Iljin K, Bourcier C, Alitalo K, Pouyssegur J, and Pages G. Endothelial cells genetically selected from differentiating mouse embryonic stem cells incorporate at sites of neovascularization in vivo. J Cell Sci 115: 2075–2085, 2002.
Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci USA 78: 7634–7638, 1981.
Martin GR and Evans MJ. The morphology and growth of a pluripotent teratocarcinoma cell line and its derivatives in tissue culture. Cell 2: 163–172, 1974.
Matsui Y, Zsebo K, and Hogan BL. Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture. Cell 70: 841–847, 1992.
McBurney MW, Jones-Villeneuve EM, Edwards MK, and Anderson PJ. Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature 299: 165–167, 1982.
McDonald JW and Howard MJ. Repairing the damaged spinal cord: a summary of our early success with embryonic stem cell transplantation and remyelination. Prog Brain Res 137: 299–309, 2002.
McDonald JW, Liu XZ, Qu Y, Liu S, Mickey SK, Turetsky D, Gottlieb DI, and Choi DW. Transplanted embryonic stem cells survive, differentiate and promote recovery in injured rat spinal cord. Nat Med 5: 1410–1412, 1999.
Metzger JM, Lin WI, and Samuelson LC. Vital staining of cardiac myocytes during embryonic stem cell cardiogenesis in vitro. Circ Res 78: 547–552, 1996.
Metzger JM, Samuelson LC, Rust EM, and Westfall MV. Embryonic stem cell cardiogenesis: applications for cardiovascular research. Trends Cardiovasc Med 7: 63–68, 1997.
Meyer N, Jaconi M, Landopoulou A, Fort P, and Puceat M. A fluorescent reporter gene as a marker for ventricular specification in ES-derived cardiac cells. FEBS Lett 478: 151–158, 2000.
Miller-Hance WC, LaCorbiere M, Fuller SJ, Evans SM, Lyons G, Schmidt C, Robbins J, and Chien KR. In vitro chamber specification during embryonic stem cell cardiogenesis. Expression of the ventricular myosin light chain-2 gene is independent of heart tube formation. J Biol Chem 268: 25244–25252, 1993.
Min JY, Yang Y, Converso KL, Liu L, Huang Q, Morgan JP, and Xiao YF. Transplantation of embryonic stem cells improves cardiac function in postinfarcted rats. J Appl Physiol 92: 288–296, 2002.
Mintz B and Illmensee K. Normal genetically mosaic mice produced from malignant teratocarcinoma cells. Proc Natl Acad Sci USA 72: 3585–3589, 1975.
Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, and Yamanaka S. The homeoprotein nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113: 631–642, 2003.
Mombaerts P. Therapeutic cloning in the mouse. Proc Natl Acad Sci USA 100: 11924–11925, 2003.
Morshead CM, Benveniste P, Iscove NN, and van der Kooy D. Hematopoietic competence is a rare property of neural stem cells that may depend on genetic and epigenetic alterations. Nat Med 8: 268–273, 2002.
Muller AM and Dzierzak EA. ES cells have only a limited lymphopoietic potential after adoptive transfer into mouse recipients. Development 118: 1343–1351, 1993.
Muller M, Fleischmann BK, Selbert S, Ji GJ, Endl E, Middeler G, Muller OJ, Schlenke P, Frese S, Wobus AM, Hescheler J, Katus HA, and Franz WM. Selection of ventricular-like cardiomyocytes from ES cells in vitro. FASEB J 14: 2540–2548, 2000.
Mullins LJ, Wilmut I, and Mullins JJ. Nuclear transfer in rodents. J Physiol Online, 2003.
Mummery C, Ward-van Oostwaard D, Doevendans P, Spijker R, van den Brink S, Hassink R, van der Heyden M, Opthof T, Pera M, d la Riviere AB, Passier R, and Tertoolen L. Differentiation of human embryonic stem cells to cardiomyocytes: role of coculture with visceral endoderm-like cells. Circulation 107: 2733–2740, 2003.
Mummery C, Ward-van Oostwaard D, van den Brink S, Bird SD, Doevendans PA, Opthof T, Brutel de la Riviere A, Tertoolen L, van der Heyden M, and Pera M. Cardiomyocyte differentiation of mouse and human embryonic stem cells. J Anat 200: 233–242, 2002.
Mummery CL, van Achterberg TA, van den Eijnden-van Raaij AJ, van Haaster L, Willemse A, de Laat SW, and Piersma AH. Visceral-endoderm-like cell lines induce differentiation of murine P19 embryonal carcinoma cells. Differentiation 46: 51–60, 1991.
Muyrers JP, Zhang Y, and Stewart AF. Techniques: recombinogenic engineering: new options for cloning and manipulating DNA. Trends Biochem Sci 26: 325–331, 2001.
Nagy A. Cre recombinase: the universal reagent for genome tailoring. Genesis 26: 99–109, 2000.
Nakano T, Kodama H, and Honjo T. Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science 265: 1098–1101, 1994.
Nakano T, Kodama H, and Honjo T. In vitro development of primitive and definitive erythrocytes from different precursors. Science 272: 722–724, 1996.
Naldini L, Blomer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, and Trono D. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272: 263–267, 1996.
Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Scholer H, and Smith A. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95: 379–391, 1998.
Nicolas JF, Dubois P, Jakob H, Gaillard J, and Jacob F. Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line. Ann Microbiol 126: 3–22, 1975.
Nishikawa SI, Nishikawa S, Hirashima M, Matsuyoshi N, and Kodama H. Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages. Development 125: 1747–1757, 1998.
Niwa H, Burdon T, Chambers I, and Smith A. Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev 12: 2048–2060, 1998.
Niwa H, Miyazaki J, and Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 24: 372–376, 2000.
Odorico JS, Kaufman DS, and Thomson JA. Multilineage differentiation from human embryonic stem cell lines. Stem Cells 19: 193–204, 2001.
Okabe S, Forsberg-Nilsson K, Spiro AC, Segal M, and McKay RD. Development of neuronal precursor cells and functional postmitotic neurons from embryonic stem cells in vitro. Mech Dev 59: 89–102, 1996.
Okuda A, Fukushima A, Nishimoto M, Orimo A, Yamagishi T, Nabeshima Y, Kuro-o M, Nabeshima Yi Boon K, Keaveney M, Stunnenberg H, and Muramatsu M. UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. EMBO J 17: 2019–2032, 1998.
Olsen JC. Gene transfer vectors derived from equine infectious anemia virus. Gene Therapy 5: 1481–1487, 1998.
Orkin SH, Porcher C, Fujiwara Y, Visvader J, and Wang LC. Intersections between blood cell development and leukemia genes. Cancer Res 59: 1784–1787, 1999.
Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, Pickel J, McKay R, Nadal-Ginard B, Bodine DM, Leri A, and Anversa P. Bone marrow cells regenerate infarcted myocardium. Nature 410: 701–705, 2001.
Pain B, Clark ME, Shen M, Nakazawa H, Sakurai M, Samarut J, and Etches RJ. Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities. Development 122: 2339–2348, 1996.
Palacios R, Golunski E, and Samaridis J. In vitro generation of hematopoietic stem cells from an embryonic stem cell line. Proc Natl Acad Sci USA 92: 7530–7534, 1995.
Pannell D, Osborne CS, Yao S, Sukonnik T, Pasceri P, Karaiskakis A, Okano M, Li E, Lipshitz HD, and Ellis J. Retrovirus vector silencing is de novo methylase independent and marked by a repressive histone code. EMBO J 19: 5884–5894, 2000.
Papaioannou VE, McBurney MW, Gardner RL, and Evans MJ. Fate of teratocarcinoma cells injected into early mouse embryos. Nature 258: 70–73, 1975.
Parisi S, D'Andrea D, Lago CT, Adamson ED, Persico MG, and Minchiotti G. Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells. J Cell Biol 163: 303–314, 2003.
Pau KY and Wolf DP. Derivation and characterization of monkey embryonic stem cells. Reprod Biol Endocrinol 2: 41, 2004.
Peckham I, Sobel S, Comer J, Jaenisch R, and Barklis E. Retrovirus activation in embryonal carcinoma cells by cellular promoters. Genes Dev 3: 2062–2071, 1989.
Pera MF, Reubinoff B, and Trounson A. Human embryonic stem cells. J Cell Sci 113: 5–10, 2000.
Perrier AL, Tabar V, Barberi T, Rubio ME, Bruses J, Topf N, Harrison NL, and Studer L. Derivation of midbrain dopamine neurons from human embryonic stem cells. Proc Natl Acad Sci USA 101: 12543–12548, 2004.
Perry D. Patients' voices: the powerful sound in the stem cell debate. Science 287: 1423, 2000.
Pesce M, Anastassiadis K, and Scholer HR. Oct-4: lessons of totipotency from embryonic stem cells. Cells Tissues Organs 165: 144–152, 1999.
Petersen BE, Bowen WC, Patrene KD, Mars WM, Sullivan AK, Murase N, Boggs SS, Greenberger JS, and Goff JP. Bone marrow as a potential source of hepatic oval cells. Science 284: 1168–1170, 1999.
Pfeifer A, Brandon EP, Kootstra N, Gage FH, and Verma IM. Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Proc Natl Acad Sci USA 98: 11450–11455, 2001.
Pfeifer A, Ikawa M, Dayn Y, and Verma IM. Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci USA 99: 2140–2145, 2002.
Pipeleers D. The biosociology of pancreatic B cells. Diabetologia 30: 277–291, 1987.
Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, and Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science 284: 143–147, 1999.
Poeschla EM, Wong-Staal F, and Looney DJ. Efficient transduction of nondividing human cells by feline immunodeficiency virus lentiviral vectors. Nature Med 4: 354–357, 1998.
Potocnik AJ, Nielsen PJ, and Eichmann K. In vitro generation of lymphoid precursors from embryonic stem cells. EMBO J 13: 5274–5283, 1994.
Powers AC, Rabizadeh A, Akeson R, and Eisenbarth GS. Characterization of monoclonal antibody 3G5 and utilization of this antibody to immobilize pancreatic islet cell gangliosides in a solid phase radioassay. Endocrinology 114: 1338–1343, 1984.
Prelle K, Vassiliev IM, Vassilieva SG, Wolf E, and Wobus AM. Establishment of pluripotent cell lines from vertebrate species: present status and future prospects. Cells Tissues Organs 165: 220–236, 1999.
Prelle K, Wobus AM, Krebs O, Blum WF, and Wolf E. Overexpression of insulin-like growth factor-II in mouse embryonic stem cells promotes myogenic differentiation. Biochem Biophys Res Commun 277: 631–638, 2000.
Proetzel G and Wiles MV. The use of a chemically defined media for the analyses of early development in ES cells and mouse embryos. Methods Mol Biol 185: 17–26, 2002.
Putnam AJ and Mooney DJ. Tissue engineering using synthetic extracellular matrices. Nat Med 2: 824–826, 1996.
Quinn G, Ochiya T, Terada M, and Yoshida T. Mouse flt-1 promoter directs endothelial-specific expression in the embryoid body model of embryogenesis. Biochem Biophys Res Commun 276: 1089–1099, 2000.
Quinonez R and Sutton RE. Lentiviral vectors for gene delivery into cells. DNA and Cell Biol 21: 937–951, 2002.
Rajagopal J, Anderson WJ, Kume S, Martinez OI, and Melton DA. Insulin staining of ES cell progeny from insulin uptake. Science 299: 363, 2003.
Ramalho-Santos M, Yoon S, Matsuzaki Y, Mulligan RC, and Melton DA. "Stemness": transcriptional profiling of embryonic and adult stem cells. Science 298: 597–600, 2002.
Rambhatla L, Chiu CP, Kundu P, Peng Y, and Carpenter MK. Generation of hepatocyte-like cells from human embryonic stem cells. Cell Transplant 12: 1–11, 2003.
Rao A. Sampling the universe of gene expression. Nature Biotechnol 16: 1311–1312, 1998.
Rathjen J, Haines BP, Hudson KM, Nesci A, Dunn S, and Rathjen PD. Directed differentiation of pluripotent cells to neural lineages: homogeneous formation and differentiation of a neurectoderm population. Development 129: 2649–2661, 2002.
Rathjen J, Lake JA, Bettess MD, Washington JM, Chapman G, and Rathjen PD. Formation of a primitive ectoderm like cell population, EPL cells, from ES cells in response to biologically derived factors. J Cell Sci 112: 601–612, 1999.
Rathjen J, Washington JM, Bettess MD, and Rathjen PD. Identification of a biological activity that supports maintenance and proliferation of pluripotent cells from the primitive ectoderm of the mouse. Biol Reprod 69: 1863–1871, 2003.
Reppel M, Boettinger C, and Hescheler J. Beta-adrenergic and muscarinic modulation of human embryonic stem cell-derived cardio-myocytes. Cell Physiol Biochem 14: 187–196, 2004.
Resnick JL, Bixler LS, Cheng L, and Donovan PJ. Long-term proliferation of mouse primordial germ cells in culture. Nature 359: 550–551, 1992.
Reubinoff BE, Itsykson P, Turetsky T, Pera MF, Reinhartz E, Itzik A, and Ben Hur T. Neural progenitors from human embryonic stem cells. Nat Biotechnol 19: 1134–1140, 2001.
Reubinoff BE, Pera MF, Fong CY, Trounson A, and Bongso A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 18: 399–404, 2000.
Reubinoff BE, Pera MF, Vajta G, and Trounson AO. Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method. Hum Reprod 16: 2187–2194, 2001.
Richards M, Fong CY, Chan WK, Wong PC, and Bongso A. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 20: 933–936, 2002.
Richards M, Tan SP, Tan JH, Chan WK, and Bongso A. The transcriptome profile of human embryonic stem cells as defined by SAGE. Stem Cells 22: 51–64, 2004.
Rideout WM, Eggan K, and Jaenisch R. Nuclear cloning and epigenetic reprogramming of the genome. Science 293: 1093–1098, 2001.
Rideout WM, Hochedlinger K, Kyba M, Daley GQ, and Jaenisch R. Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy. Cell 109: 17–27, 2002.
Risau W, Sariola H, Zerwes HG, Sasse J, Ekblom P, Kemler R, and Doetschman T. Vasculogenesis and angiogenesis in embryonic-stem-cell-derived embryoid bodies. Development 102: 471–478, 1988.
Robertson E, Bradley A, Kuehn M, and Evans M. Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector. Nature 323: 445–448, 1986.
Robertson EJ. Embryo-derived stem cell lines. In: Teratocarcinoma and Embryonic Stem Cells: a Practical Approach, edited by E. J. Robertson. Oxford, UK: IRL, 1987, p. 71–112.
Rodda SJ, Kavanagh SJ, Rathjen J, and Rathjen PD. Embryonic stem cell differentiation and the analysis of mammalian development. Int J Dev Biol 46: 449–458, 2002.
Rodda S, Sharma S, Scherer M, Chapman G, and Rathjen P. CRTR-1, a developmentally regulated transcriptional repressor related to the CP2 family of transcription factors. J Biol Chem 276: 3324–3332, 2001.
Rogers MB, Hosler BA, and Gudas LJ. Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes. Development 113: 815–824, 1991.
Rohwedel J, Guan K, Hegert C, and Wobus AM. Embryonic stem cells as an in vitro model for mutagenicity, cytotoxicity and embryotoxicity studies: present state and future prospects. Toxicol In Vitro 15: 741–753, 2001.
Rohwedel J, Guan K, and Wobus AM. Induction of cellular differentiation by retinoic acid in vitro. Cells Tissues Organs 165: 190–202, 1999.
Rohwedel J, Guan K, Zuschratter W, Jin S, Ahnert-Hilger G, Furst D, Fassler R, and Wobus AM. Loss of beta1 integrin function results in a retardation of myogenic, but an acceleration of neuronal, differentiation of embryonic stem cells in vitro. Dev Biol 201: 167–184, 1998.
Rohwedel J, Horak V, Hebrok M, Fuchtbauer EM, and Wobus AM. M-twist expression inhibits mouse embryonic stem cell-derived myogenic differentiation in vitro. Exp Cell Res 220: 92–100, 1995.
Rohwedel J, Maltsev V, Bober E, Arnold HH, Hescheler J, and Wobus AM. Muscle cell differentiation of embryonic stem cells reflects myogenesis in vivo: developmentally regulated expression of myogenic determination genes and functional expression of ionic currents. Dev Biol 164: 87–101, 1994.
Rohwedel J, Sehlmeyer U, Shan J, Meister A, and Wobus AM. Primordial germ cell-derived mouse embryonic germ (EG) cells in vitro resemble undifferentiated stem cells with respect to differentiation capacity and cell cycle distribution. Cell Biol Int 20: 579–587, 1996.
Rolletschek A, Chang H, Guan K, Czyz J, Meyer M, and Wobus AM. Differentiation of embryonic stem cell-derived dopaminergic neurons is enhanced by survival-promoting factors. Mech Dev 105: 93–104, 2001.
Rosler ES, Fisk GJ, Ares X, Irving J, Miura T, Rao MS, and Carpenter MK. Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dyn 229: 259–274, 2004.
Ryding ADS, Sharp MGF, and Mullins JJ. Conditional transgenic technologies. J Endocrinol 171: 1–14, 2001.
Sato N, Meijer L, Skaltsounis L, Greengard P, and Brivanlou AH. Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med 10: 55–63, 2004.
Sato N, Sanjuan IM, Heke M, Uchida M, Naef F, and Brivanlou AH. Molecular signature of human embryonic stem cells and its comparison with the mouse. Dev Biol 260: 404–413, 2003.
Sauer H, Gunther J, Hescheler J, and Wartenberg M. Thalidomide inhibits angiogenesis in embryoid bodies by the generation of hydroxyl radicals. Am J Pathol 156: 151–158, 2000.
Schnell T, Foley P, Wirth M, Munch J, and Uberla K. Development of a self-inactivating, minimal lentivirus vector based on simian immunodeficiency virus. Hum Gene Ther 11: 439–447, 2000.
Schöler HR, Hatzopoulos AK, Balling R, Suzuki N, and Gruss P. A family of octamer-specific proteins present during mouse embryogenesis: evidence for germline-specific expression of an Oct factor. EMBO J 8: 2543–2550, 1989.
Scholz G, Pohl I, Genschow E, Klemm M, and Spielmann H. Embryotoxicity screening using embryonic stem cells in vitro: correlation to in vivo teratogenicity. Cells Tissues Organs 165: 203–211, 1999.
Schoonjans L, Albright GM, Li JL, Collen D, and Moreadith RW. Pluripotential rabbit embryonic stem (ES) cells are capable of forming overt coat color chimeras following injection into blastocysts. Mol Reprod Dev 45: 439–443, 1996.
Schoonjans L, Kreemers V, Danloy S, Moreadith RW, Laroche Y, and Collen D. Improved generation of germline-competent embryonic stem cell lines from inbred mouse strains. Stem Cells 21: 90–97, 2003.
Schuldiner M, Eiges R, Eden A, Yanuka O, Itskovitz-Eldor J, Goldstein RS, and Benvenisty N. Induced neuronal differentiation of human embryonic stem cells. Brain Res 913: 201–205, 2001.
Schuldiner M, Yanuka O, Itskovitz-Eldor J, Melton DA, and Benvenisty N. From the cover: effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells. Proc Natl Acad Sci USA 97: 11307–11312, 2000.
Segev H, Fishman B, Ziskind A, Shulman M, and Itskovitz-Eldor J. Differentiation of human embryonic stem cells into insulin-producing clusters. Stem Cells 22: 265–274, 2004.
Shamblott MJ, Axelman J, Littlefield JW, Blumenthal PD, Huggins GR, Cui Y, Cheng L, and Gearhart JD. Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro. Proc Natl Acad Sci USA 98: 113–118, 2001.
Shamblott MJ, Axelman J, Wang S, Bugg EM, Littlefield JW, Donovan PJ, Blumenthal PD, Huggins GR, and Gearhart JD. Derivation of pluripotent stem cells from cultured human primordial germ cells. Proc Natl Acad Sci USA 95: 13726–13731, 1998.
Sharov AA, Piao Y, Matoba R, Dudekula DB, Qian Y, VanBuren V, Falco G, Martin PR, Stagg CA, Bassey UC, Wang Y, Carter MG, Hamatani T, Aiba K, Akutsu H, Sharova L, Tanaka TS, Kimber WL, Yoshikawa T, Jaradat SA, Pantano S, Nagaraja R, Boheler KR, Taub D, Hodes RJ, Longo DL, Schlessinger D, Keller J, Klotz E, Kelsoe G, Umezawa A, Vescovi AL, Rossant J, Kunath T, Hogan BL, Curci A, D’Urso M, Kelso J, Hide W, and Ko MS. Transcriptome analysis of mouse stem cells and early embryos. PloS Biology, 1: E74, 2003.
Shibata H, Toyama K, Shioya H, Ito M, Hirota M, Hasegawa S, Matsumoto H, Takano H, Akiyama T, Toyoshima K, Kanamaru R, Kanegae Y, Saito I, Nakamura Y, Shiba K, and Noda H. Rapid colorectal adenoma formation initiated by conditional targeting of the APC gene. Science 278: 120–123, 1997.
Shiroi A, Yoshikawa M, Yokota H, Fukui H, Ishizaka S, Tatsumi K, and Takahashi Y. Identification of insulin-producing cells derived from embryonic stem cells by zinc-chelating dithizone. Stem Cells 20: 284–292, 2002.
Sipione S, Eshpeter A, Lyon JG, Korbutt GS, and Bleackley RC. Insulin expressing cells from differentiated embryonic stem cells are not beta cells. Diabetologia 47: 499–508, 2004.
Skarnes WC, Auerbach BA, and Joyner AL. A gene trap approach in mouse embryonic stem cells: the lacZ reported is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice. Genes Dev 6: 903–918, 1992.
Skarnes WC, Moss JE, Hurtley SM, and Beddington RSP. Capturing genes encoding membrane and secreted proteins important for mouse development. Proc Natl Acad Sci USA 92: 6592–6596, 1995.
Smith AG. Embryo-derived stem cells: of mice and men. Annu Rev Cell Dev Biol 17: 435–462, 2001.
Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, and Rogers D. Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. Nature 336: 688–690, 1988.
Solter D, Beyleveld D, Friele MB, Holowka J, Lilie H, Lovell-Badge R, Mandla C, Martin U, and Pardo Avellaneda R. Embryo Research in Pluralistic Europe. Berlin: Springer-Verlag, 2003.
Solter D and Knowles BB. Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proc Natl Acad Sci USA 75: 5565–5569, 1978.
Soria B. In-vitro differentiation of pancreatic beta-cells. Differentiation 68: 205–219, 2001.
Soria B, Roche E, Berna G, Leon-Quinto T, Reig JA, and Martin F. Insulin-secreting cells derived from embryonic stem cells normalize glycemia in streptozotocin-induced diabetic mice. Diabetes 49: 157–162, 2000.
Sperger JM, Chen X, Draper JS, Antosiewicz JE, Chon CH, Jones SB, Brooks JD, Andrews PW, Brown PO, and Thomson JA. Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors. Proc Natl Acad Sci USA 100: 13350–13355, 2003.
Spielmann H, Pohl I, Doering B, Liebsch M, and Moldenhauer F. The embryonic stem cell test, an in vitro embryotoxicity test using two permanent mouse cell lines: 3T3 fibroblasts and embryonic stem cells. Toxicol In Vitro 10: 119–127, 1997.
Spielmann H, Scholz G, Pohl I, Genschow E, Klemm M, and Visan A. The use of transgenic embryonic stem (ES) cells and molecular markers of differentiation for improving the embryonic stem cell test (EST). Congenital Anomalies 40: 8–18, 2000.
Stanford WL, Caruana G, Vallis KA, Inamdar M, Hidaka M, Bautch VL, and Bernstein A. Expression trapping: identification of novel genes expressed in hematopoietic and endothelial lineages by gene trapping in ES cells. Blood 92: 4622–4631, 1998.
Stern MD, Anisimov SV, and Boheler KR. Can transcriptome size be estimated from SAGE catalogs? Bioinformatics 19: 443–448, 2003.
Stevens LC. Origin of testicular teratomas from primordial germ cells in mice. J Natl Cancer Inst 38: 549–552, 1967.
Stevens LC. The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev Biol 21: 364–382, 1970.
Stevens LC. The origin and development of testicular, ovarian, and embryo-derived teratomas. In: Teratocarcinoma Stem Cells, edited by L. M. Silver, G. R. Martin, and S. Strickland. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1983, p. 23–36.
Stewart CL, Gadi I, and Bhatt H. Stem cells from primordial germ cells can reenter the germ line. Dev Biol 161: 626–628, 1994.
Stewart CL, Stuhlmann H, Jahner D, and Jaenisch R. De novo methylation, expression, and infectivity of retroviral genomes introduced into embryonal carcinoma cells. Proc Natl Acad Sci USA 79: 4098–4102, 1982.
Stojkovic M, Lako M, Stojkovic P, Stewart R, Przyborski S, Armstrong L, Evans J, Herbert M, Hyslop L, Ahmad S, Murdoch A, and Strachan T. Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture. Stem Cells 22: 790–797, 2004.
Strubing C, Ahnert-Hilger G, Shan J, Wiedenmann B, Hescheler J, and Wobus AM. Differentiation of pluripotent embryonic stem cells into the neuronal lineage in vitro gives rise to mature inhibitory and excitatory neurons. Mech Dev 53: 275–287, 1995.
Suda Y, Suzuki M, Ikawa Y, and Aizawa S. Mouse embryonic stem cells exhibit indefinite proliferative potential. J Cell Physiol 133: 197–201, 1987.
Suemori H, Tada T, Torii R, Hosoi Y, Kobayashi K, Imahie H, Kondo Y, Iritani A, and Nakatsuji N. Establishment of embryonic stem cell lines from cynomolgus monkey blastocysts produced by IVF or ICSI. Dev Dyn 222: 273–279, 2001.
Sutton J, Costa R, Klug M, Field L, Xu D, Largaespada DA, Fletcher CF, Jenkins NA, Copeland NG, Klemsz M, and Hromas R. Genesis, a winged helix transcriptional repressor with expression restricted to embryonic stem cells. J Biol Chem 271: 23126–23133, 1996.
Svoboda P, Stein P, Hayashi H, and Schultz RM. Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference. Development 127: 4147–4156, 2000.
Takeshima H, Komazaki S, Hirose K, Nishi M, Noda T, and Iino M. Embryonic lethality and abnormal cardiac myocytes in mice lacking ryanodine receptor type 2. EMBO J 17: 3309–3316, 1998.
Tanaka TS, Kunath T, Kimber WL, Jaradat SA, Stagg CA, Usuda M, Yokota T, Niwa H, Rossant J, and Ko MSH. Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity. Genome Res 12: 1921–1928, 2002.
Tang F, Shang K, Wang X, and Gu J. Differentiation of embryonic stem cell to astrocytes visualized by green fluorescent protein. Cell Mol Neurobiol 22: 95–101, 2002.
Terada N, Hamazaki T, Oka M, Hoki M, Mastalerz DM, Nakano Y, Meyer EM, Morel L, Petersen BE, and Scott EW. Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion. Nature 416: 542–545, 2002.
Testa G, Zhang YM, Vintersten K, Benes V, Pijnappel WWMP, Chambers I, Smith AJH, Smith AG, and Stewart AF. Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles. Nature Biotechnol 21: 443–447, 2003.
Thomas KR and Capecchi MR. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51: 503–512, 1987.
Thompson S, Clarke AR, Pow AM, Hooper ML, and Melton DW. Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells. Cell 56: 313–321, 1989.
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, and Jones JM. Embryonic stem cell lines derived from human blastocysts. Science 282: 1145–1147, 1998.
Thomson JA, Kalishman J, Golos TG, Durning M, Harris CP, Becker RA, and Hearn JP. Isolation of a primate embryonic stem cell line. Proc Natl Acad Sci USA 92: 7844–7848, 1995.
Thomson JA, Kalishman J, Golos TG, Durning M, Harris CP, and Hearn JP. Pluripotent cell lines derived from common marmoset (Callithrix jacchus) blastocysts. Biol Reprod 55: 254–259, 1996.
Toyooka Y, Tsunekawa N, Akasu R, and Noce T. Embryonic stem cells can form germ cells in vitro. Proc Natl Acad Sci USA 100: 11457–11462, 2003.
Tropepe V, Hitoshi S, Sirard C, Mak TW, Rossant J, and van der Kooy D. Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism. Neuron 30: 65–78, 2001.
Troy TC and Turksen K. ES cell differentiation into the hair follicle lineage in vitro. Methods Mol Biol 185: 255–260, 2002.
Tsai M, Wedemeyer J, Ganiatsas S, Tam SY, Zon LI, and Galli SJ. In vivo immunological function of mast cells derived from embryonic stem cells: an approach for the rapid analysis of even embryonic lethal mutations in adult mice in vivo. Proc Natl Acad Sci USA 97: 9186–9190, 2000.
Turksen K. Embryonic Stem Cells: Methods and Protocols. Totowa, NJ: Humana, 2002.
Usdin S. Ethical issues associated with pluripotent stem cells. In: Human Embryonic Stem Cells, edited by C. Y. Chiu and M. S. Rao. Totowa, NJ: Humana, 2003, chapt. 1, p. 3–25.
Vacanti JP and Langer R. Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation. Lancet 354: SI32–SI34, 1999.
Vassilieva S, Guan K, Pich U, and Wobus AM. Establishment of SSEA-1- and Oct-4-expressing rat embryonic stem-like cell lines and effects of cytokines of the IL-6 family on clonal growth. Exp Cell Res 258: 361–373, 2000.
Velculescu VE, Zhang L, Vogelstein B, and Kinzler KW. Serial analysis of gene expression. Science 270: 484–487, 1995.
Ventura C, Zinellu E, Maninchedda E, and Maioli M. Dynorphin B is an agonist of nuclear opioid receptors coupling nuclear protein kinase C activation to the transcription of cardiogenic genes in GTR1 embryonic stem cells. Circ Res 92: 623–629, 2003.
Viswanathan S, Frishman LJ, and Robson JG. The uniform field and pattern ERG in macaques with experimental glaucoma: removal of spiking activity. Invest Ophthalmol Vis Sci 41: 2797–2810, 2000.
Von Melchner H, DeGregori JV, Rayburn H, Reddy S, Friedel C, and Ruley HE. Selective disruption of genes expressed in totipotent embryonal stem cells. Genes Dev 6: 919–927, 1992.
Vrana KE, Hipp JD, Goss AM, McCool BA, Riddle DR, Walker SJ, Wettstein PJ, Studer LP, Tabar V, Cunniff K, Chapman K, Vilner L, West MD, Grant KA, and Cibelli JB. Nonhuman primate parthenogenetic stem cells. Proc Natl Acad Sci 100: 11911–11916, 2003.
Wagers AJ, Sherwood RI, Christensen JL, and Weissman IL. Little evidence for developmental plasticity of adult hematopoietic stem cells. Science 297: 2256–2259, 2002.
Wagers AJ and Weissman IL. Plasticity of adult stem cells. Cell 116: 639–648, 2004.
Wang R, Clark R, and Bautch VL. Embryonic stem cell-derived cystic embryoid bodies form vascular channels: an in vitro model of blood vessel development. Development 114: 303–316, 1992.
Wartenberg M, Gunther J, Hescheler J, and Sauer H. The embryoid body as a novel in vitro assay system for antiangiogenic agents. Lab Invest 78: 1301–1314, 1998.
Wassarman PM and Keller GM. Differentiation of Embryonic Stem Cells. New York: Elsevier, 2003.
Watt FM and Hogan BL. Out of Eden: stem cells and their niches. Science 287: 1427–1430, 2000.
Weissman IL, Anderson DJ, and Gage F. Stem and progenitor cells: origins, phenotypes, lineage commitments, and transdifferentiations. Annu Rev Cell Dev Biol 17: 387–403, 2001.
Weitzer G, Milner DJ, Kim JU, Bradley A, and Capetanaki Y. Cytoskeletal control of myogenesis: a desmin null mutation blocks the myogenic pathway during embryonic stem cell differentiation. Dev Biol 172: 422–439, 1995.
Wernig M, Tucker KL, Gornik V, Schneiders A, Buschwald R, Wiestler OD, Barde YA, and Brüstle O. Tau EGFP embryonic stem cells: an efficient tool for neuronal lineage selection and transplantation. J Neurosci Res 69: 918–924, 2002.
Whitney M, Rockenstein E, Cantin G, Knapp T, Zlokarnik G, Sanders P, Durick K, Craig FF, and Negulescu PA. A genome-wide functional assay of signal transduction in living mammalian cells. Nature Biotechnol 16: 1329–1333, 1998.
Wianny F and Zernicka-Goetz M. Specific interference with gene function by double-stranded RNA in early mouse development. Nature Cell Biol 2: 70–75, 2000.
Wichterle H, Lieberam I, Porter JA, and Jessell TM. Directed differentiation of embryonic stem cells into motor neurons. Cell 110: 385–397, 2002.
Wiles MV and Keller G. Multiple hematopoietic lineages develop from embryonic stem (ES) cells in culture. Development 111: 259–267, 1991.
Williams RL, Hilton DJ, Pease S, Willson TA, Stewart CL, Gearing DP, Wagner EF, Metcalf D, Nicola NA, and Gough NM. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336: 684–687, 1988.
Wilmut I, Schnieke AE, McWhir J, Kind AJ, and Campbell KH. Viable offspring derived from fetal and adult mammalian cells. Nature 385: 810–813, 1997.
Wilson JM. Animal models of human disease for gene therapy. J Clin Invest 97: 1138–1141, 1996.
Wobus AM, Guan K, Shan J, Wellner MC, Rohwedel J, Ji G, Fleischmann B, Katus HA, Hescheler J, and Franz WM. Retinoic acid accelerates embryonic stem cell-derived cardiac differentiation and enhances development of ventricular cardiomyocytes. J Mol Cell Cardiol 29: 1525–1539, 1997.
Wobus AM, Guan K, Yang HT, and Boheler K. Embryonic stem cells as a model to study cardiac, skeletal muscle, and vascular smooth muscle cell differentiation In: Methods in Molecular Biology: Embryonic Stem Cells: Methods and Protocols, edited by K. Turksen. Totowa, NJ: Humana, 2002, vol. 185, chapt. 13, p. 127–156.
Wobus AM, Holzhausen H, Jakel P, and Schoneich J. Characterization of a pluripotent stem cell line derived from a mouse embryo. Exp Cell Res 152: 212–219, 1984.
Wobus AM, Rohwedel J, Maltsev V, and Hescheler J. In vitro diffferentiation of embryonic stem cells into cardiomyocytes or skeletal muscle cells is specifically modulated by retinoic acid. Roux's Arch Dev Biol 204: 36–45, 1994.
Wobus AM, Wallukat G, and Hescheler J. Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and Ca2+ channel blockers. Differentiation 48: 173–182, 1991.
Wu X, Ding S, Ding Q, Gray NS, and Schultz PG. Small molecules that induce cardiomyogenesis in embryonic stem cells. J Am Chem Soc 126: 1590–1591, 2004.
Xian HQ, McNichols E, St. Clair A, and Gottlieb DI. A subset of ES-cell-derived neural cells marked by gene targeting. Stem Cells 21: 41–49, 2003.
Xu C, Inokuma MS, Denham J, Golds K, Kundu P, Gold JD, and Carpenter MK. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19: 971–974, 2001.
Xu C, Police S, Rao N, and Carpenter MK. Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells. Circ Res 91: 501–508, 2002.
Xu RH, Chen X, Li DS, Li R, Addicks GC, Glennon C, Zwaka TP, and Thomson JA. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol 20: 1261–1264, 2002.
Yamada T, Yoshikawa M, Kanda S, Kato Y, Nakajima Y, Ishizaka S, and Tsunoda Y. In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green. Stem Cells 20: 146–154, 2002.
Yamamoto H, Quinn G, Asari A, Yamanokuchi H, Teratani T, Terada M, and Ochiya T. Differentiation of embryonic stem cells into hepatocytes: biological functions and therapeutic application. Hepatology 37: 983–993, 2003.
Yamashita J, Itoh H, Hirashima M, Ogawa M, Nishikawa S, Yurugi T, Naito M, Nakao K, and Nishikawa S. Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors. Nature 408: 92–96, 2000.
Yanagimachi R. Cloning: experience from the mouse and other animals. Mol Cell Endocrinol 187: 241–248, 2002.
Yang HT, Tweedie D, Wang S, Guia A, Vinogradova T, Bogdanov K, Allen PD, Stern MD, Lakatta EG, and Boheler KR. The ryanodine receptor modulates the spontaneous beating rate of cardiomyocytes during development. Proc Natl Acad Sci USA 99: 9225–9230, 2002.
Yang S, Tutton S, Pierce E, and Yoon K. Specific double-stranded RNA interference in undifferentiated mouse embryonic stem cells. Mol Cell Biol 21: 7807–7816, 2001.
Ying QL, Nichols J, Chambers I, and Smith A. BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115: 281–292, 2003.
Ying QL, Stavridis M, Griffiths D, Li M, and Smith A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol 21: 183–186, 2003.
Zambrowicz BP, Abuin A, Ramirez-Solis R, Richter LJ, Piggott J, BeltrandelRio H, Buxton EC, Edwards J, Finch RA, and Friddle CJ. Wnk1 kinase deficiency lowers blood pressure in mice: a gene-trap screen to identify potential targets for therapeutic intervention. Proc Natl Acad Sci USA 100: 14109–14114, 2003.
Zamore PD, Tuschl T, Sharp PA, and Bartel DP. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 101: 25–33, 2000.
Zandstra PW, Le HV, Daley GQ, Griffith LG, and Lauffenburger DA. Leukemia inhibitory factor (LIF) concentration modulates embryonic stem cell self-renewal and differentiation independently of proliferation. Biotechnol Bioeng 69: 607–617, 2000.
Zhang SC, Wernig M, Duncan ID, Brüstle O, and Thomson JA. In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotechnol 19: 1129–1133, 2001.
Zheng B, Sage M, Sheppeard EA, Jurecic V, and Bradley A. Engineering mouse chromosomes with Cre-loxP: range, efficiency, and somatic applications. Mol Cell Biol 20: 648–655, 2000.
Zlokarnik G, Negulescu PA, Knapp TE, Mere L, Burres N, Feng LX, Whitney M, Roemer K, and Tsien RY. Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter. Science 279: 84–88, 1998.
Zou GM, Wu W, Chen JJ, and Rowley JD. Duplexes of 21-nucleotide RNAs mediate RNA interference in differentiated mouse ES cells. Biol Cell 95: 365–371, 2003.
Zwaka TP and Thomson JA. Homologous recombination in human embryonic stem cells. Nat Biotechnol 3: 319–321, 2003.
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