Figures
- Dengue virus type 2: replication and tropisms in orally infected Aedes aegypti mosquitoes

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Figure 1. DENV-2 midgut infection (MIR) and dissemination rates (DR) in Aedes aegypti mosquitoes. MIRs and DRs for Chetumal, Rex-D, and D2S3 mosquito strains at 14 dpi as described in Materials and Methods. The mean and standard deviation values were obtained from four independent experiments (n= 50–60 mosquitoes).

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Figure 2. Kinetics of DENV-2 midgut infection after oral challenge of Aedes aegypti. Chetumal mosquito midguts dissected at different time points (n = 30 for each time point) were assayed by IFA to detect DENV-2 viral antigen (green). At each time point two representative midguts from Chetumal mosquitoes are presented. Controls are an uninfected midgut and a dengue infected midgut (in red midgut musculature). The magnification was 100×.

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Figure 3. Quantification of DENV-2 RNA and infectious virus in midguts revealed significant differences. Midguts (n = 15) from orally infected Chetumal mosquitoes were assayed to quantify viral RNA by Q-PCR and infectious particles by end point titrations at different time points. A) Viral RNA decreased slightly between 7 and 14 dpi and then remained stable, * P B) Viral titers declined significantly at 14 and 21 dpi, *** P

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Figure 4. DENV-2 antigen in the tracheal system of Chetumal mosquitoes following infection. DENV-2 antigen was detected by IFA in trachea of Chetumal mosquitoes at 2, 3, 4, 5, and 7 dpi (n= 40–50). Midgut- associated tracheal system (A) and uninfected trachea using light (B) and fluorescence (C) microscopy. Green arrows point to portions of DENV-2 infected trachea (E-L) (200×).

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Figure 5. DENV-2 tropism for Aedes aegypti salivary glands. Salivary glands were dissected at the time points noted, fixed, and assayed for virus antigen by IFA (n = 30–50). Representative salivary glands (200×) from Chetumal and Rex-D mosquitoes at each time point are shown. DENV-2 virus antigen increased in the salivary glands through out the EIP. Negative controls (A and B) and salivary glands examined with light microscopy (C) are shown. In Rex-D mosquitoes, at 4 dpi viral antigen was typically associated with the fat body surrounding the salivary glands. In contrast salivary gland tissues of Chetumal mosquitoes contained viral antigen at 4 dpi.

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Figure 6. DENV-2 tropisms in different tissues and organs of Aedes aegypti. DENV-2 viral E antigen distribution in different tissues and organs was revealed by IFA (green for FITC or Alexa 488) in Chetumal mosquitoes (n = 20). A) DENV-2 antigen in fat body of mosquito abdomen at 2 dpi. B) DENV-2 infected epithelial cells in midgut (4 dpi). C) DENV-2 is present in epithelial cells but absent in midgut-associated muscle (red phalloidin-Alexa^®546). D) DENV-2 in anterior midgut at 5 dpi. E) Infected esophagus at 7 dpi, dr.dv. dorsal diverticulum, car: cardia, oe: esophagus, and cr: crop. F) Hemocytes infected with DENV-2 at 10 dpi. G) Ommatidia of the compound eye exhibiting viral antigen at 12 dpi. H) Nervous system tissue profusely infected by DENV-2 at 14 dpi. I) Malphigian tubules expressing viral antigen (Mal. Tu.) at 16 dpi, il: ileum. These pictures represent the infection patterns observed. Original magnification was 200×, but pictures were cropped in Adobe Photoshop to improve presentation.

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Figure 7. Temporal tropisms of DENV-2 in Chetumal mosquitoes. Infection rates in the respective organ or tissue are shown by the gray scale. Percentages were obtained after dissecting, staining and analyzing individual mosquitoes (n = 20–30) at each time point. Mosquitoes were assayed at 1, 2, 3, 5, 7, 10, 14, 18 and 21 dpi.

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Figure 8. Kinetics of DENV-2 replication in orally infected mosquitoes. DENV-2 titers in individual Chetumal mosquitoes were determined by plaque assay at 3, 4, 7, 10, 14, 18 and 21 dpi. Each box in the graph represents mean and range values of viral titers (n = 10) in mosquitoes for each time point.