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In this study, we used culture and culture-independent methods to determine the …

Biology Articles » Microbiology » Culture-independent analysis of bacterial diversity in a child-care facility » Tables

Tables
- Culture-independent analysis of bacterial diversity in a child-care facility

Table 1
Unique morphologies present in culture results, and their identification through sequencing
Organism
 Sequence Length1
 % Identity2
 GenBank Accesssion
 Morphology
Acinetobacter sp.
 741
 98
 EF409307
 Acinet. 1
Bacillus licheniformis
 720
 99
 EF409308
 Bacillus M1
Bacillus megaterium
 739
 99
 EF409309
 Bacillus M2
Bacillus sp.
 498
 97
 EF409310
 Bacillus M3
Bacillus sp.
 754
 100
 EF409311
 Bacillus M4
Bacillus sp.
 724
 99
 EF409312
 Bacillus M 5 (Nutrient)
Bacillus sp.
 748
 99
 EF409313
 Bacillus M6
Bacillus sp.
 730
 100
 EF409314
 Bacillus M7
Bacillus sp.
 664
 99
 EF409315
 Bacillus M8
Bacillus sp.
 741
 89
 EF409316
 Bacillus M9
Bacillus sp.
 677
 98
 EF409317
 Bacillus M10
Bacillus sp.
 721
 99
 EF409318
 Bacillus M11
Bacillus subtilis
 759
 99
 EF409319
 Bacillus M12
Bacillus subtilis
 759
 100
 EF409320
 Bacillus M5a
Bacillus subtilis
 736
 99
 EF409321
 Bacillus M13
Bacillus subtilis
 722
 99
 EF409322
 Bacillus M14
Bacillus subtilis
 718
 99
 EF409323
 Bacillus M15
Brevibacillus sp.
 711
 99
 EF409324
 Brevi. M1a
Brevibacterium sp.
 695
 99
 EF409325
 Brevi. M1b
Enterococcus faecalis
 764
 99
 EF409326
 Enterococ. M1
Exiguobacterium sp.
 767
 99
 EF409327
 Exiguobac. M1
Microbacterium aurum
 289
 99
 EF409328
 Microbac. M1a
Microbacterium esteraromaticum
 327
 98
 EF409329
 Microbac. M1b
Moraxella osloensis
 750
 98
 EF409330
 Morax. M1
Pseudomonas stutzeri
 682
 99
 EF409331
 Pseudom. M1
Staphylococcus aureus
 752
 99
 EF409332
 Staph. M1
Staphylococcus aureus
 727
 100
 EF409333
 Staph. M2
Staphylococcus epidermidis
 721
 100
 EF409334
 Staph. M3
Staphylococcus haemolyticus
 703
 99
 EF409335
 Staph. M4
1 Length of sequence used in BLAST search
2 Based on BLAST alignment
3 Abbreviations for each morphology type; cross-referenced in Table 2
Table 2
Culture results for environmental swab samples
Date
 Plate type
 Total Plates
 % Growth1
 Organisms detected2
30-Sep-2005
 Nutrient
 6
 66.7
 N/A

Blood
 4
 75.0
 N/A
6-Oct-2005
 Nutrient
 12
 0.0
 N/A

Blood
 12
 33.3
 N/A
14-Oct-2005
 Nutrient
 14
 16.7
 N/A

Blood
 0
 0.0
 N/A
21-Oct-2005
 Nutrient
 24
 79.2
 Bacillus sp (Bacillus M7)

Blood
 0
 0.0
 Bacillus sp. (Bacillus M2, M11)
28-Oct-2005
 Nutrient
 10
 80.0
 N/A

Blood
 6
 83.3
 N/A
3-Nov-2005
 Nutrient
 13
 84.6
 Bacillus sp. (Bacillus M7)

Blood
 12
 83.3
 Bacillus sp. (Bacillus M11)
15-Nov-2005
 Nutrient
 22
 81.8
 Bacillus sp. (Bacillus M7)

Blood
 16
 56.3
 Bacillus sp. (Bacillus M3, M11, 14)
18-Jan-2006
 Nutrient
 16
 56.3
 N/A

Blood
 0
 0.0
 N/A
25-Jan-2006
 Nutrient
 16
 68.8
 N/A

Blood
 16
 75.0
 N/A
3-Feb-2006
 Nutrient
 12
 75.0
 Bacillus sp. (Bacillus M2, M7, M10), Staphylococcus sp. (Staph. M1)

Blood
 16
 81.3
 Bacillus sp. (Bacillus M1, M2, M3, M14), Staphylococcus sp. (Staph. M2), Moraxella sp. (Morax. M1), Brevibacterium sp. (Brevi. M1b), Brevibacillus sp. (Brevi. M1a)
15-Feb-2006
 Nutrient
 10
 90.0
 Bacillus sp. (Bacillus M10), Staphylococcus sp. (Staph. M3)

Blood
 10
 80.0
 Bacillus sp. (Bacillus M1), Microbacterium sp. (Microbac. M1)
1-Mar-2006
 Nutrient
 10
 70.0
 Pseudomonas sp. (Pseudo. M1), Bacillus sp. (Bacillus M1, M5, M7), Staphylococcus sp. (Staph M3)

Blood
 10
 60.0
 Bacillus sp. (Bacillus M1, M5, M9, M11), Staphylococcus sp. (Staph. M2)
8-Mar-2006
 Nutrient
 20
 85.0
 Bacillus sp. (Bacillus M5, M7), Staphylococcus sp. (Staph. M3), Enterococcus sp. (Enterococ. M1)

Blood
 20
 90.0
 Bacillus sp. (Bacillus M1, M11), Staphylococcus sp. (Staph. M2), Moraxella sp. (Morax. M1)
29-Mar-2006
 Nutrient
 16
 50.0
 Bacillus sp. (Bacillus M5, M7), Staphylococcus sp. (M1), Enterococcus sp. (Enterococ. M1)

Blood
 16
 50.0
 Bacillus sp. (Bacillus M2, M5a, M11, M13), Staphylococcus sp. (M2)
5-Apr-2006
 Nutrient
 12
 41.7
 Bacillus sp. (Bacillus M7, M12), Staphylococcus sp. (Staph. M1, M3), Acinetobacter sp. (Acinet. M1)

Blood
 12
 58.3
 Bacillus sp. (Bacillus M2, M8, M11, M14), Staphylococcus sp. (M2, M3)
12-Apr-2006
 Nutrient
 16
 37.5
 Bacillus sp. (Bacillus M7, M12), Brevibacterium sp. (Brevi. M1b), Pseudomonas sp. (Pseudo. M1), Exiguobacterium sp. (Exiguobac. M1)

Blood
 16
 43.8
 Bacillus sp. (Bacillus M1, M2, M8, M11), Staphylococcus sp. (Staph. M2), Brevibacterium sp. (Brevi. M1a), Exiguobacterium sp. (Exiguobac. M1)
19-Apr-2006
 Nutrient
 16
 43.8
 Bacillus sp. (Bacillus M10, M12), Acinetobacter sp. (Acinet. M1), Enterococcus sp. (Enterococ. M1)

Blood
 16
 81.3
 Bacillus sp. (Bacillus M1, 2, M15), Brevibacterium sp. (Brevi. M1b), Microbacterium sp. (Microbac. M1a)
1 Percentage of plates with microbial growth
2 Observed morphologies (see Table 1); N/A = Not Available for that sampling day.

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