Tables
- A cricket Gene Index: a genomic resource for studying neurobiology, speciation, and molecular evolution

Table 1

Sequencing results of the two libraries which were examined including raw sequencing results and acceptable sequences after removing poor quality reads and contaminating sequences.

	Pooled LK libraries	Library LK01	Library LK04
EST Sequence Total Reads	all reads	5' end reads only	3' end reads	all reads	5' end reads only	3' end reads	all reads	5' end reads only	3' end reads

Number of Successful Sequences	14502	14261	241	388	316	72	14114	13945	169
Range in Length	241–1252	268–1252	241–1128	758–1150	958–1150	758–1102	241–1252	268–1252	241–1128
Mean Length	1057	1058	1024	1082	1092	1041	1057	1057	1017
High Quality EST Reads
Number of Successful Sequences	14502	14261	241	388	316	72	14114	13945	169
Range in Length	64–1096	64–1096	66–1051	68–1074	218–1074	68–943	64–1096	64–1096	66–1051
Mean Length	838	841	619	805	875	499	838	840	670
EST Sequence After Vector Stripping
Number of Successful Sequences	14377	14158	219	354	295	59	14023	13863	160
Range in Length	100–1051	100–949	103–1051	100–926	100–926	105–916	100–1051	100–949	103–1051
Mean Length	704	705	657	486	473	553	710	710	695

Table 2

Statistics of Tentative Consensus sequences (TCs)

Number of TC	2575
Number of ESTs assembled into TC	8345
TC size range (bp)	167–3317
Mean TC length (bp)	935
Range of number of EST's in TC	2–41
Average number of EST's in TC	3.24
Number of TC with >= 20 EST's	17
Number of TC with	2205

Table 3

Statistics of singletons

Number of singletons	6032
Singleton size range (bp)	102–1019
Mean singleton length (bp)	700
Number of singletons	110
Number of singletons between 200 and 500 bp	505
Number of singletons between 500 and 800 bp	3860
Number of singletons > 800 bp	1557

Table 4

Genes of neurobiological interest

Sequence ID	Gene

TC1375	Calmodulin
1099956307901	Calpain B
1099956293105	cAMP-dependent protein kinase subunit R2 beta
1099956429052	cGMP-dependent protein kinase
TC588	cGMP-gated cation channel protein
TC140	Diapause bioclock protein
TC1309	Even-Skipped
1099956350726	G-protein-coupled receptor
1099817827099	Innexin
1099817862791	Intersectin-1
TC1333	Membrane-associated ring finger
1099956579253	MscS Mechanosensitive ion channel
1099956736101	Myosin V
1099956378602	Na+/K+/2Cl-cotransporter
TC1855	Nicotinic acetylcholine receptor non-alpha subunit precursor
TC2167	Notch
1099956498166	Period
TC1283	Potassium channel tetramerisation domain-containing protein 5
1099956317550	Rab7
TC1866	Ras-related protein Rab-2
1099956329054	Serpentine Receptor
TC1295	Shab-related delayed-rectifier K+ channel
1099956378537	sodium and chloride-dependent high-affinity choline transporter
TC456	Sparc
TC2021	Stathmin
1099817880653	Swelling dependent chloride channel
1099817832930	Syntaxin 7
1099956598763	Troponin T
TC2416	Voltage-dependent anion channel
1099956851891	Wnt inhibitory factor 1

Table 5

Genes of comparative interest. Uncorrected distances between Laupala and the specified taxon are shown, where possible. The mean uncorrected pairwise distance (p) between all taxa (excluding Laupala) is shown for each gene in the final column for comparison. Alignments of each gene are presented as NEXUS files in the online additional files.

	*Locusta*	*Tribolium*	*Apis*	*Bombyx*	*Anopheles*	*Drosophila*	*Mean Distance (excluding Laupala)*

Actin	0.0911	0.1752	0.1262	0.1594	0.1051	0.0911	0.1368
Alpha-tubulin	0.2090	0.2143	0.2288	0.1744	0.2135	0.1878	0.2115
Aquaporin	0.3164	0.4715	0.4242	0.4814	0.4400	0.4336	0.4485
Dynein (Light Chain)	0.1741	0.2482	0.1741	0.6043	0.2185	0.2037	0.2111
Histone 2A	0.3184	0.2720	0.3081	0.2478	0.2016	0.3218	0.3039
HSP40	0.3959	0.4832	0.3592	0.3392	0.3587	0.4049	0.4287
Malate Esterase	0.3056	0.4032	0.3526	-	0.4140	0.4430	0.3802
Myosin 2 (Light Chain)	0.2576	0.3529	0.3132	0.3352	0.4254	0.3856	0.3652
Opsin	0.3430	-	0.3630	-	0.4173	0.4387	0.3911
Polyubiquitin	0.2046	0.2292	0.2237	0.2046	0.2846	0.2194	0.2321

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Tables - A cricket Gene Index: a genomic resource for studying neurobiology, speciation, and molecular evolution

Tables
- A cricket Gene Index: a genomic resource for studying neurobiology, speciation, and molecular evolution